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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 51
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
C
erberin (2-o-Acetyl neriifolin) is the principal cardiac glycoside
present in the seeds of
Cerbera odollam
belonging to the
Apocyanaceae family. The seeds of
Cerbera odollam
are used as a
poison for suicidal as well as homicidal purpose by people around
the world. Its detection in the body fluids is somewhat difficult.
The aim of this study was to develop a FT-IR spectrophotometric
procedure for the analysis of cerberin in rat plasma3. A Fourier
transform infrared (FT-IR) spectrometric method was developed
for the rapid, direct determination of cerberin in rat plasma.
The universal ATR spectra was recorded and used for this
study. Multiple linear regressions (MLR), with a restricted set of
absorption band were used for calibration. Beer-Lambert law was
used for data processing. A recovery of 98.8% of cerberin from rat
plasma with a correlation coefficient of 0.9980 was obtained. The
linear regression equation for cerberin was calculated to be y =
-1.0943–1.5875 x, where x and y are concentration and integrated
peak area, respectively. The method had excellent reproducibility
for the standard of 0.2 mg, 0.19±0.107% (n=6). The recovery test
is an experimental design to verify the relationship between the
amount of substance added and the amount quantified by this
assay. In this test, the observed concentrations of pure cerberin
in rat plasma were not significantly different from the stated
concentrations by Student’s t-test, P=0.05% (100.06±1.28%, n=3).
The method gave rise to linear data in the range 0.1–0.8 mg
with accuracy and precision in the range 0.86–1.4%. Therefore,
this FT-IR-spectrophotometric assay was accurate, and may be
recommended for the simple quantification of cerberin.
Recent Publications
1. Prasanth S S and Rajasekaran A (2015) Derivative
ultra-violet spectroscopic method for the estimation of
cerberin in rat plasma. International Journal of Pharma
and Bio Sciences 6(1):749 –758.
2. Prasanth S S and Rajasekaran A (2015) Visible
spectrophotometric determination of cerberin in rat
plasma. J App Pharm Sci, 5(03):109–112.
3. Prasanth S S and Aiyalu R (2015) Quantitative
determination of cerberin in seed extract of
Cerbera
odollam
and rat serum by high performance thin layer
chromatography. J App Pharm Sci. 5(3):061–069.
4. Carlier J, Guitton J, Bevalot F, Fanton L and Gaillard
Y (2014) The principal toxic glycosidic steroids in
Cerbera
manghas L. seeds: identification of cerberin,
nerifolin, tanhinin and deacetyltanghinin by UHPLC-
HRMS/MS, quantification by UHPLC-PDA-MS. Journal
of Chromatography Analytical Technology Biomed Life
Sciences 962:1–8.
5. Yvan Gaillard, Ananthasankaran Krishnamoorthy and
Fabien Bevalota (2004)
Cerbera odollam
: a ‘suicide tree’
and cause of death in the state of Kerala, India. Journal of
Ethnopharmacology 95:123–126.
FTIR-spectrophotometric analysis of cerberin in rat plasma
Prasanth S S
1
and
Rajasekaran A
2
1
Al Shifa College of Pharmacy, India
2
KMCH College of Pharmacy, India
Prasanth S S et al., Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-005