38 / 38
Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 59
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
X
anthium
strumarium
L. is poisonous to mammals due its toxic
principle which is a diterpenoid glycoside i.e., atractyloside
found in the roots and seeds. It was thought worthwhile to
carry out the hepatotoxic assessments and safety and toxicity
evaluations of oral administration of atractyloside andmethanolic
extracts of
X. strumarium
L. in albino Wistar rats. So, the
present investigation was undertaken with following objectives:
to develop standardized protocols for extraction, isolation,
purification, characterization and quantitative estimation of
atractyloside; hepatotoxic assessments of oral administration
of atractyloside in albino Wistar rats and to study the safety and
toxicity evaluation of methanolic extract in albino Wistar rats.
Xanthium
strumarium
Linn. roots and seeds were found to contain
alkaloids, anthraquinones, flavonoids, atractyloside, phenolics,
steroids, terpenoids, and resin etc. In the present investigation, an
attempt was made to separate the atractyloside by using instant
preparative thin layer chromatography (IPTLC) technique. Purified
atractyloside was chemically characterized by IR, mass and NMR
spectral analysis. Atractyloside concentrations were found to
be 2.9 and 4.3 mg/ml in plant root and seeds respectively using
HPLC techniques. During hepatotoxic assessment, atractyloside
produced severe hepatotoxicity inalbinoWistar rats. Observations
of the sub-acute and acute toxicity studies had indicated that
methanolic extract of
X. strumarium
had shown a narrow safety
margin in animals. On the basis of sub-acute and acute toxicity
evaluation studies, it was established that both atractyloside
and methanolic extract of
X. strumarium
L. possess a narrow
safety margin in rats used in
in vivo
experimental and pre-clinical
pharmacological studies.
Biography
Bhanu P S Sagar completed his PhD from Jamia Hamdard, Postdoctorate
from National Institute of Immunology .Presently, he is the Director of Phar-
macy College at IEC-GI and Former Vice-Chancellor of IEC University. He has
published 47 papers and presented 30 papers. He has also presented two
papers in AAPS 2006, National Biotechnology Conference in Boston, USA.
He is the Reviewer for various international journals and was also selected
for “Marquis Who’s Who in Asia” and “Marquis Who’s Who in World”. He has
receivedmany awards in his prime areas of research include plant tissue cul-
ture, phytochemical and pharmacological investigations of natural products
bpssagar@gmail.comSafety and toxicity evaluations of Xanthium strumarium Linn
Bhanu P S Sagar
and
Srishti Singh
IEC Group of Institutions, India
Bhanu P S Sagar et al., Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-005