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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 57
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Statement of the Problem:
Bacillary dysentery which is caused
by one or more types of
Shigella
species, highly prevalent in hot
countries with poor sanitation like Sudan especially in children
is a potentially serious disorder if left untreated. It can lead
to dehydration in children or liver abscess if it is spread from
intestine to the liver stream. Options of treating Shigellosis
with antimicrobials are becoming limited because of globally
emerging antimicrobial resistance and the long use of these
synthetic drugs causing many side effects. Therefore, the
search for effective and safer antimicrobial agents from plants
has become an area of interest in active research. The purpose
of this study is to investigate the antimicrobial activity of the
ethanol extracts of
Peganum harmala
and
Trachyspermum ammi
against isolated
Shigella flexneri and Shigella dysenteriae
causing
bacillary dysentery.
Methodology:
T. ammi
and
P. harmala
were extracted by 96%
ethanol using Soxhlet apparatus. The antimicrobial activity of the
extracts was investigated according to the disc diffusion method
and the minimum inhibitory concentrations of the extracts were
determined.
Findings:
All tested bacteria (
Shigella flexneri and Shigella
dysenteriae
) were found to be sensitive against
T. ammi
seed
extracts, while the same bacteria were resistant for amoxicillin
and amoxicillin + clavulanic acid and insensitive for P. harmala.
The tested bacteria were also sensitive to gentamycin 5 mg/ml,
but it has a lower inhibition zone than that of T. ammi 100 mg/ml.
C
onclusion & Significance:
T. ammi
ethanolic extract possessed
antimicrobial activity as stated in literature.
Recommendations:
Further investigations should be carried out
to find the bioactive compounds for developing new antibiotic.
Recent Publications
1. Darabpour E, Motamedi H, Poshtkouhian Bavi A, Nejad S
and Mansour S (2011) Antibacterial activity of different
parts of
Peganum harmala
L. growing in Iran against
multi-drug resistant bacteria. Excli Journal 10:52–263.
2. Mukherjee P K, Kumar V, Kumar N S and Heinrich M
(2008). The Ayurvedic medicine Clitoria ternatea from
traditional use to scientific assessment. Journal of
Ethnopharmacology 120(3):291–301.
3. Niyogi S K (2005) Shigellosis. Journal of Microbiology
43(2):133–143.
4. Vasudevan K, Vembar S, Veeraraghavan K and Haranath
P S (1999) Influence of intragastric perfusion of aqueous
spice extracts on acid secretion in anesthetized albino
rats. Indian Journal of Gastroenterology 19(2):53–56.
5. Choudhury S, Ahmed R, Kanjilal P B and Leclercq P A
(1998) Composition of the seed oil of
Trachyspermum
ammi
(L.) Sprague from Northeast India. Journal of
Essential Oil Research 10(5):588–590.
Biography
IbrahimSiddigHamid has his expertise in evaluation and passion in research,
search and discovery of natural drugs from plant origin. His open and con-
textual evaluation model based on responsive constructivists creates new
drugs for improving and treating of infectious diseases
ibrahimsiddig95@gmail.comIn vitro susceptibility of isolated Shigella flexneri and Shigella
dysenteriae to the ethanolic extracts of Trachyspermum ammi
and Peganum harmala
Ibrahim Siddig Hamid
and
Ikram Mohamed Eltayeb
University of Medical Sciences and Technology, Sudan
Ibrahim Siddig Hamid et al., Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-005