29 / 38
Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 50
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Objective:
The current study investigated the antiurolithiatic
effect of some fractions of methanolic whole plant extract of
Rubia tinctorum
(RT) in rats.
Methods:
The RT was successively extracted with, ethyl
acetate, butanol and water to obtain fractions. Calcium oxalate
urolitihiasis was induced in rats by ethylene glycol–ammonium
chloride feeding in drinking water for 10 days. These rats were
treated with two doses (1000 and 2000 mg/kg) of the fractions.
Antiurolithiatic activity was assessed by estimating biochemical
changes in serum, urine and histological changes in kidney tissue.
Results:
Sodium oxalate administration caused biochemical
alterations in urine which was found to be prevented significantly
by the butanolic and ethyl acetate fractions. Supplementation
with butanolic and ethyl acetate fraction prevented the elevation
of serum creatinine, urea, uric acid, phosphor and calcium levels.
The butanolic and ethyl acetate fractions also caused significant
decrease in lipid peroxidation activity, accumulation of calcium
oxalate deposits and histological changes in the kidney tissue.
Conclusion:
The results showed that the antiurolithiatic
component of the methanolic
Rubia tinctorum
extract of the plant
is contained in the butanolic and ethyl acetate fractions. The
effect is associated to its diuretic, antioxidant, nephroprotective
properties and effect on lowering the concentration of urinary
stone-forming constituents.
marhoume.fatimazahra@gmail.comProtective effect of butanolic and ethyl acetate fractions
of Rubia tinctorum extract against calcium oxalate induced
urolithiasis in rats
F Marhoume
1, 2
, A Alioua
3
, H Boufous
1, 2
, Y Zaid
4
, A Chait
2
and
A Bagri
1
1
Université Hassan I, Morocco
2
Université Cadi Ayyad, Morocco
3
Mohammed V University, Morocco
4
Mohammed VI University of Health Sciences, Morocco
F Marhoume et al., Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-005