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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 54
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Background:
Mycetoma is a devastating, slow-growing bacterial
or fungal infection, which develops into a chronic infection of the
skin tissues and if left untreated, eventually results in amputation.
With existing anti-fungal and surgery, only 35% of people living
with the fungal form of the disease, known as eumycetoma, are
cured,
Madurella mycetomatis
has been found to shield itself
against the antifungal therapy through the production of melanin
decreasing the effectiveness of the therapy,
Arctostaphylos uva-
ursi
has proven antimicrobial effect and is known for it’s content of
arbutin which is a potent inhibitor of melanin synthesis in humans,
can hopefully potentiate the action of the present therapy and
optimize its effect. The purpose of this study is to investigate the
effect of arbutin powder and
A. uva-ursi
aqueous leaf extract on
synthesis of melanin by
Madurella mycetomatis
.
Methodology & Theoretical Orientation:
Aqueous extract of
A.
uva-ursi
was prepared by dissolving 50 g of
A. uva-ursi
leaves in
1000 ml of water:methanol ratio of 95:5. The arbutin solution was
prepared by dissolving 0.5 g of arbutin powder in 20 ml of sterile
distilledwater. The study of the effect of aqueous extract of
A. uva-
ursi
and arbutin on the synthesis of melanin by
M. mycetomatis
was carried out as described by van de Sande.
Findings:
The inhibitory effect of arbutin on melanin synthesis
by
M. mycetomatis
was found to be dose dependent.
A. uva-
ursi
aqueous leaf extract containing arbutin was also found to
decrease melanin production by M.
mycetomatis
.
Conclusion & Significance:
Inhibition of melanin synthesis was
observed in the arbutin powder as well as the aqueous extract
containing arbutin
Recent Publications
1. Elfadil H, Fahal A, KloezenW, AhmedEMandvandeSande
W (2015). The
in vitro
antifungal activity of Sudanese
medicinal plants against
Madurella mycetomatis
, the
eumycetoma major causative agent. PLoS Negl Trop Dis.
9(3):e0003488.
2. Van de Sande W W, de Kat J, Coppens J, Ahmed
A O, Fahal A, et al. (2007) Melanin biosynthesis in
Madurella mycetomatis
and its effect on susceptibility to
itraconazole and ketoconazole. Microbes and Infection
9(9)1114–1123.
3. Van de Sande W W J, Fahal A, Verbrugh H and Belkum
Al (2007) Polymorphisms in genes involved in innate
immunity predispose toward mycetoma susceptibility.
Journal of Immunology 179:3065–3074.
4. Ahmed A, Adelmann D, Fahal A, Verbrugh H, van Belkum
A, et al. (2002) Environmental occurrence of
Madurella
mycetomatis
, the major agent of human eumycetoma
in Sudan. Journal of Clinical Microbiology 40(3):1031–
1036.
aminaomer9@gmail.comThe effect of arbutin powder and Arctostaphylos uvaursi
aqueous leaf extract on synthesis of melanin by Madurella
mycetomatis
Amina Saadeldin Abdelmotalib Omer
and
Ikram Mohamed Eltayeb
University of Medical Sciences and Technology, Sudan
Amina Saadeldin Abdelmotalib Omer et al., Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-005