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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 101
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006
P
soriasis is a chronic, relapsing inflammatory skin disease,
characterized by erythematous plaques covered by silvery
scales. While the pathogenesis of psoriasis is still unclear, most
findings indicated that intensifying T helper 1(Th1) and Th17
are required for the development of psoriasis. Cucurbitacin
D is chemically classified as steroid with anticancer activity
on endometrial and ovarian cancer cells. First, we evaluated
the effects of cucurbitacin D on inflammatory mediator and
chemokine production in IL-1α, IL-17A, IL-22, oncostatin M, and
TNF-α (named M5 combination)-stimulated 3D reconstituted
human epidermis. Cucurbitacin D significantly inhibited the
production of IL-1β, IL-20 and GM-CSF. Second, we examined
whether cucurbitacin D influences the expression of proliferation
marker in IL-22 stimulated HaCaT keratinocytes. Cucurbitacin
D significantly suppressed the expression of K16, K17 and Ki67
in IL-22 stimulated HaCaT cells at a concentration of 30µM.
Cucurbitacin D also suppressed M5 induced phosphorylation of
ERK 1/2 in HaCaT cells. These results suggest that cucurbitacin
D may be useful as an anti-inflammatory agent, especially for
psoriasis.
heungmuk@dongguk.ac.krCucurbitacin D inhibited pro-inflammatory cytokine production
and proliferation in keratinocytes
Heung Mook Shin
and
In Jun Yang
Dongguk University, South Korea