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Pharmacognosy 2018 American Journal of Ethnomedicine
ISSN: 2348-9502 Page 98
April 16-17, 2018
Amsterdam, Netherlands
6th Edition of International Conference on
Pharmacognosy and
Medicinal Plants
T he objective of this study was to investigate the acute toxicity
and potential activity of methanolic extract of Hyoscyamus
muticus (Me-HM) to assess nociception in mice. The acute
toxicity was studied in both oral and intraperitoneal route. LD50
was determined using Probit method and the effect of extract
against nociceptive was studied by thermal stimulus (hot plate)
and injection of chemical substances such as formalin (formalin
test) and acetic acid (writhing test). Morphine was used as
positive drug in hotplate test and acetylsalicylic acid was used
in formalin and writhing test. The antinociceptive activity was
determined by observed increase of latency time in hotplate
test, decrease of abdominal constriction in writhing test and
decrease of stretching in formalin test. The LD50 of intraperitonial
administration of Me-HM was 1000±42, 89 mg/kg-1. Our extract
produced a significant (P≤0.001) and dose dependent increase of
latency time in hotplate test. The optimal effects were observed
after 90 mins of oral administration of both doses. In the formalin
test, the both doses reduce significantly (P≤0.001) the effect
produced by intraplantar injection of formalin with maximum
inhibition recorded in neurogenic phase with 49.36% and 42.67%
successively for 100 and 50 mg/kg of Me-HM. Morphine and
acetylsalicylic acid produced desired anti-nociceptive activity in
tests used in this study. The antinociceptive effect of H. muticus
extract can be explained probably by binding of scopolamine and
hyoscyamine from extract to muscarinic receptors and 5- HT3
involved in pain pathways. It was concluded that Me-HM shows
a remarkable antinociceptive activity in thermal and chemical
model of nociception in mice.
Acute toxicity and anti-nociceptive activity of methanolic
extract of Hyoscyamus muticus in mice
Hicham Boufous1, Fatimazahra Marhoume 1, Abderrahman Chait2 and Abdellah Bagri1
1Hassan 1st University, Morocco
2Cadi Ayyad University, Morocco
Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006