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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 106
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
P
harmacological and phytochemical research shows that
Combretum fragrans
F. Hoffm (Combretaceae) is a plant
possessing numerous therapeutic virtues and rich in active
secondary compounds. In this study, we investigate the
in vivo
(antinociceptive, anti-inflammatory) and
in vitro
(anti-cancer,
anti-TNFα, ROS and NO inhibitor) capacity of Combretin A and
Combretin B, the triterpenes (cycloartane-type) isolated from
Combretum fragrans
. ROS production from phagocytes, TNF-α
production, NO production, anticancer and cytotoxicity assay
were done by using chemiluminescence technique, ELISA kit,
colorimetric method, MCF-7 Cells and MTT assay, respectively.
Antinociceptive and anti-inflammatory activity was estimated
usingamodel of acetic acid, formalinandcarrageenan. Combretin
A and Combretin B significantly (p<0.001) inhibited extracellular
ROS production. These compounds also possess significant
(p<0.001) reduced TNF-α and NO production. The compound
decreased cell viability in MCF-7 cells. Concerning the pain
induced by acetic acid and formalin as acute inflammation in rat
induced by carrageenan, Combretin A and Combretin B exhibited
significantly (p<0.001) antinociceptive and anti-inflammatory
activity. Antinociceptive, anti-inflammatory and anticancer
potential associated with inhibitors effect on ROS, TNFα and NO
production revealed in this study approve that, Combretin A and
Combretin B are considered as a promising chemotherapeutic
agent in breast cancer treatment and inflammatory disease.
mbiantchamarius@yahoo.frAnalgesic, anti-inflammatory and anticancer activities
of Combretin A and Combretin B isolated from Combretum
fragrans F. Hoffm (Combretaceae) leaves
Mbiantcha Marius, Almas jabeen, Dawe Amadou, Faheem Aisha
and
Sidra Zafar
Dschang, Cameroon
Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006