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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 112
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Background:
Doxorubicin(Dox)isahighlyactivechemotherapeutic
drug used to treat several solid and hematologic tumors. However,
its clinical use is limited by its severe cardiotoxic side effects
including, reactive oxygen species (ROS) production. The aim of
this study is to investigate the protective effects of plants extracts
used as spices against the Dox-induced toxicity on H9c2 cells and
elucidates their mechanism of action.
Methodology:
The cytoprotective effects of the extracts of
Afrostyrax lepidophyllus (GEH) and Monodora myristica (AEH)
were tested by assessing the growth and viability of H9c2
cells treated with doxorubicin in presence of the extracts using
sulforodamine B (SRB) and resazurine assays. The effects of
extracts on morphological and biochemical changes of the cells
were explored through the mitochondrial membrane potential
(ΔΨm), the production of ROS, and the activity of caspases 3 and
9.
Results:
The SRB assay showed that the samples AEH
(60.56±9.83%) and GEH (65.26±9.29%) at the concentration of
25 µg/mL have revealed a significant (P<0.05) protective effect
of cell growth and the resazurine assay confirmed their effect on
cell viability with the respective values of 68.94 ± 6.00% and 74.68
± 5.84% that were significantly higher compared to the control
treated with Dox. We found an increase of the ΔΨm in presence
of the samples: GEH (68.75±7.99%) and AEH (68.41±3.21%)
compared to the group receiving the Dox (56.91±4.18%). Also
we noted a decrease production of ROS in living H9C2 cells
and reduction of the activity of the caspases 3 and 9. The
morphological assessment showed a reduction of apoptotic
cells, nucleus fragmentation and the TMRE showed an increase
of the ΔΨm in the presence of AEH and GEH.
Conclusion & Significance:
AEH and GEH extracts demonstrated
protective effect against the deleterious effects of Dox on
cardiomyocytes. Their mechanism implied the reduction of
apoptosis in cardiac cells and oxidative stress. Therefore, they
could be used as inhibitor of Dox cardiotoxicity to improve cancer
treatment.
apieme@yahoo.frCardioprotective effect of two Cameroonian spices against
doxorubicin induced cardiotoxicity on H9C2 cells
Pieme C A
1
, Moukette M B
1,2
, Ferreira L
2
, Simões R
2
, Deus C
2
, Silva Am
2
, Sardão V
2
and
Oliveira Paulo J
2
1
University of Yaoundé I, Cameroon
2
University of Coimbra, Portugal
Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006