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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 96
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Statement of theProblem:
Parinari curatellifolia (Planch ex. Benth
Chrysobalanaceae) is used in traditional medicine in Nigeria for
the treatment of various diseases including microbial infections.
The aim of the present study was to determine the potentials of
the total crude saponins (TCS), extracted from the root bark of the
plant against some selected pathogenic fungi and bacteria.
Methodology & Theoretical Orientation:
The plant was collected
from Zaria and authenticated at the Department of Biological
Sciences, Ahmadu Bello University, Zaria, Nigeria. The TCS was
extracted using standard procedures and was subjected to
qualitative phytochemical tests. The antibacterial activities of
TCS were evaluated against clinical isolates of Staphylococcus
aureus, Bacillus subtilis,
Escherichia coli,
and Pseudomonas
aeruginosa using the cup-plate method. The TCS demonstrated
strong antibacterial activitieswith zones of inhibition (in diameter)
ranging between 8.0 and 21.0 mm, showing concentration-
dependent responses on each of the test microorganisms.
Staphylococcus aureus was the most susceptible producing the
longest zone of inhibition (21.0 mm) at 30 mg/mL with MIC and
MBC of 3.75 mg/mL, indicating that the TCS was bactericidal
on
S. aureus
. Similarly, the antifungal activities of the TCS were
also evaluated using the agar diffusion cup-plate method against
clinical isolates of Trichophyton mentagrophytes, Trichophyton
rubrum and Aspergillus niger with typed isolates of Candida
albican (ATCC 102311). TCS showed zones of inhibition ranging
from 12–18 mm at 140 mg/mL on Trichophyton mentagrophytes,
Trichophyton rubrumand Aspergillus niger. AnMIC90 andMFC90
of 195 µg/mL were obtained for Aspergillus niger using themicro-
broth dilution. This investigation revealed that the total crude
saponins possessed fungicidal effect on Aspergillus niger. There
was no activity observed on Candida albican.
Conclusion & Significance:
This study has revealed both
antifungal and antibacterial properties for the total crude
saponins, extracted from Parinari curatellifolia root bark.
Recommendations:
Further work should be conducted on
the separation, isolation, characterization and evaluation of
antimicrobial activity of the pure saponin(s) from this plant.
emshelia2002@yahoo.comAntimicrobial activities of total crude saponins from Parinari
curatellifolia planch ex. Benth root bark
Halilu M E, Ifeanyi G I
and
Ungokore H Y
Usmanu Danfodiyo University, Nigeria
Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006