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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 102
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
T
he plant
Conyza sumatrensis
is used in traditional medicine in
parts of Africa for the treatment of numerous health ailments
like inflammatation, skin diseases as well as cancers. Bioassay
guided fractionation of the methanol extract of the leaves
of
Conyza sumatrensis
against breast cancer (MCF-7), lung
cancer (NCI-H460) and NIH 3T3 (mouse embryonic fibroblast
normal cell line) at 1-250 µg/mL was carried out. Fractions and
isolated compounds were as well tested at 1-100 µg/mL and
1-100 µM against the cell lines. Extracts of
C. sumatrensis
was
partitioned into aqueous and chloroform fractions and both
fractions were tested for their effects on MCF-7 and NCI-H460.
Further chromatographic and biological studies of the active
chloroform fraction yielded two compounds whose identities
were revealed as Stigmasterol 3-O-beta-D-glucoside (A) and
2, 3-dihydroxylpropyl hexacosanoate (B) through NMR and MS
studies. These compounds were observed to give –16.50±0.14
and –21.71±0.23% cytotoxicities against MCF-7 at 100 µM
with GI50 and TGI of 40±0.10, 50±6.0 µM and 22.67±1.33 and
69.33±1.33 µM respectively. These compounds were also
cytotoxic against NCI-H460 celll lines but less than doxorubicin,
the anticancer drug used. The overall results showed that the
plant can be used to prevent the proliferation of breast and lung
cancer cells and hence justify the ethnomedicinal uses of the
plants in treating tumour related ailments.
ikpefanemmanuel@delsu.edu.ngIsolation and characterization of anticancer bioactive
compounds from leaves of Conyza sumatrensis, a plant reputed
for anticancer activities
Ikpefan Emmanuel O
1
, Ayinde B A
2
, Omeje E O
3
and
Choudhary M I
4
1
Delta State University, Nigeria
2
University of Benin, Nigeria
3
University of Nigeria, Nsukka, Nigeria
4
University of Karachi, Pakistan
Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006