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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 100
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Statement of the Problem:
The prevalence of NAFLD is estimated
as 25.24% and it is increasing globally. NAFLD is a progressive
disease leading to fibrosis, cirrhosis and carcinoma. Terminalia
arjunaW. & Arn. (Combretaceae) is an endemic tree found in India
and Sri Lanka and used traditionally for its cardioprotective and
hepatoprotective effects. Arjunolic acid (AA) is a triterpenoid
found majorly in the heart wood of
T. arjuna
.
Methodology &Theoretical Orientation:
AAwas isolated from the
heart wood of
T. arjuna
by the method of King et al. The structure
of AA was confirmed using spectroscopic studies. Steatosis was
induced to Hep G2 cells using FFAmixture and the effect of AA on
triglyceride accumulation and lipotoxicity were assessed.
In vivo
effect of AA on NAFLD was assessed using high fat diet fed rats.
Findings:
The treatment with AA showed GI50 value of 746.34 µM
toHepG2 cells. The treatment withAAsignificantly lowered the oil
red O concentration by 26.44% and triglyceride accumulation by
58.96% at 100 µM concentration. Fenofibrate treatment showed
29.15 and 57.56% reductions, respectively. The treatment with
AA and fenofibrate showed 74.90 and 73.28% reduction in the
leakage of LDH, compared to the vehicle treated group. The
in vivo
findings clearly demonstrated that the animals treated with AA at
50 and 100 mg/kg concentrations showed a significant decrease
in the levels of transaminases, phosphatases and LDH levels.
Further, the treatment increased the albumin to globulin ratio. The
liver histology of the treated animals was normal (Figure 1). The
treatment with AA, significantly upregulated the expression of
genes viz., PPAR-α, FXR-α and CPT-1.
Conclusion:
These evidences suggested that AA might be a
promising lead to treat NAFLD. Future robust scientific studies
on AA will shed more light on its usefulness for the treatment of
NAFLD.
eriloyola@hotmail.comCurative effect of arjunolic acid from Terminalia arjuna W.
& Arn. on cellular and rodent models of non-alcoholic fatty
liver disease
Ignacimuthu S, Toppo E, Darwin S S
and
Pandikumar P
Entomology Research Institute, India
Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006