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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 72
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5
t h
I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
A
llergen exposure during prenatal or postnatal period may alter
immune programming and affect the fate of infant’s allergic
disease. However, the relationship and mechanism between prenatal
allergen exposure and the development of allergic disease is still
unclear. We aimed to investigate whether prenatal allergen exposure
induces immune tolerance or sensitization to allergen. The peritoneal
cavity of each FVB/N fetus was directly injected with different doses
of adjuvant-free ovalbumin (OVA) or normal saline (NS) on day 14 of
gestation. Eight weeks after the birth, i
n utero
NS- and OVA-injected
mice were challenged by inhaling OVA aerosols. I
n utero
OVA-injected
adult mice with OVA challenge manifested significant induction of
airway hyperresponsiveness, lung eosinophilia, serum levels of OVA-
specific antibodies and Th2 cytokines of OVA-stimulated splenocytes.
These mice also developed serious anaphylactic reactions following
intraperitoneal injection of OVA. To further understand the mechanisms
of OVA-induced hyper-immune responsiveness, we analyzed the
OVA-specific immunity and gene expression of lungs and spleens
in prenatal OVA-exposed neonates. I
n utero
OVA-injected neonates
already had dominant OVA-specific humoral and cell-mediated
immunity. Cytokine expression pattern in the lungs of i
n utero
OVA-
injected neonates evidently favoured Th2-biased immune responses.
Furthermore, splenocytes of i
n utero
OVA-injected neonates expressed
higher RNA levels of Notch ligands (Jagged1 and Jagged2). Inhibition
of notch signalling by γ-secretase inhibitor significantly reduced OVA-
induced Th2 cytokine production and proliferative responses
in vitro
.
The results suggested that intervention of allergen exposure or notch
signalling during pregnancy may be beneficial for modulating the
development of allergic asthma.
Fetal ovalbumin exposure resulting in murine hyper-immune
responsiveness
Cheng-Chi Chan
1
, Jeng-Chang Chen
2
and Ming-Ling Kuo
1
1
Chang Gung University, Taiwan
2
Chang Gung Children’s Hospital, Taiwan
Biography
Cheng-Chi Chan has accomplished his PhD degree in 2016 fromGraduate
Institute of Biomedical Sciences, Chang Gung University. Postdoctoral
training is being performed in the Department of Microbiology
and Immunology, Chang Gung University. His studies focus on the
mechanisms among prenatal or postnatal allergen exposure and allergic
asthma development. He has a good training in the field of Immunology
and Molecular Biology and a great skill in the asthmatic animal model
and related experiments. Simultaneously, He is familiar with the operation
of intra-utero injection and the investigation of the development and
differentiation of various immune cells. The related experimental results
had been published in reputed journals.
achi8848@gmail.comCheng-Chi Chan et al., Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-003