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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 68
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5 t h I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
Antigen-presenting cells are important for the induction of immune
responses. Dendritic cells (DCs) are most effective antigen-
presenting cells. They patrol in the peripheral and continuously sample
their surrounding environment with various receptors. Fcγ receptors
(FcγRs) express on dendritic cells. They bind to the constant fragment
of IgG and are crucial to mediate internalization of antigen-antibody
complexes (immune complexes, ICs). Internalization of IC facilitates
antigen uptake and presentation by DCs. In this context, targeting of
antigen to DCs via FcγRs potentially constitutes an effective strategy
for modulation of antigen-specific immune responses. Formyl peptide
receptor-like 1inhibitory protein (FLIPr), secreted by Staphylococcus
aureus , is a potent FcγR antagonist and bind to various FcγRs. In this
study, we developed a novel antigen delivery system by fusion antigen
with FLIPr. Ovalbumin (OVA) was used as a model antigen. Our results
show that OVA-FLIPr fusion protein (OVA-FLIPr) possesses FcγR
binding ability. Immunization of mice with OVA-FLIPr but not OVA
can induce both OVA-specific CD4 and CD8 T cell responses without
exogenous adjuvant formulation. In addition, we demonstrate that
OVA-specific cytotoxicity is elicited and mediate antitumor response
in mice immunized with OVA-FLIPr. These results indicate that FLIPr
is a potential vector to deliver antigen to DCs via FcγRs which induce
robust immune responses without exogenous adjuvant formulation.
A novel antigen delivery system via Fc γ receptors induces
robust immune responses
Chen-Yi Chiang1, Yi-Jyun Chen 1, Chiao-Chieh Wu1 , Shih-Jen
Liu1,2, Chih-Hsiang Leng1,2 and Hsin-Wei Chen1,2
1National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Taiwan
2Graduate Institute of Biomedical Sciences, China Medical University, Taiwan
Biography
Hsin-Wei Chen obtained his PhD in the field of Agricultural Chemistry from
National Taiwan University, Postdoctoral training was in the immunology
field at Academia Sinica and National Health Research Institutes. He is
an Associate Investigator of National Health Research Institutes. He has
published more than 50 papers in reputed journals and has been serving
as an Editorial Board Member of repute.
Hsin-Wei Chen et al., Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-003