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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 73
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5
t h
I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
S
taphylococcus
aureus
is one of the most pathogenic organisms
which cause infections in both healthcare and community settings.
The proportion of methicillin-resistant S.
aureus
(MRSA) in healthcare-
associated (HA) isolates was very high in South Korea. In this study,
antimicrobial resistance profiles of
S. aureus
and meats isolated
bacteria were determined by disc diffusion method. PCR was applied
for detecting the presence of antibiotic resistance genes and blaTEM
types. In total of 18
S. aureus
and 36meats isolated bacterias. All strains
carried blaTEM gene which encodes an enzyme belongs to extended-
spectrum β-lactamases (ESBLs), conferring significant penicillin
resistance. MRSA strains which carried type II SCC
mec
element
presenting characteristic of multidrug resistance (MDR), these strains
harbored resistance genes ant(4’)-Ia and
ermA
, which showed positive
correlation with kanamycin and erythromycin resistance, respectively.
Genes
aac(6’)-aph(2”)
and
tetM
were also found in these strains which
phenotypically associated with gentamicin and tetracycline resistance.
In addition, type IVA was the most prevalent SCC
mec
element in our
study, which carried a variant class B
mec
complex. The J3 region of
type IVA element may integrate a copy of plasmid pUB110, harboring
ant(4’)-Ia
which phenotypically associated with kanamycin resistance.
The molecular characteristics of MRSA strains in our study are unique
compared with isolates which have spread internationally. This is the
report to show the presence of blaTEM in meats isolated bacterias.
Molecular identification of ESBL-producing gene blaTEM in
MRSA and meats isolated bacteria
Yuan Li, Yong Hyun Lee, Seok Chan Hong, Dae Young Kim, Jae
Kweon Park and You Jin Hwang
Gachon University, South Korea
Biography
Hwang has completed his PhD from Inha University. He is the director
of RIS, a Bio Health solution. He has published more than 50 papers in
reputed journals.
gene@gachon.ac.krYou Jin Hwang et al., Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-003