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Pharma 2018
Page 47
E u r o p e a n C o n g r e s s o n
Pharma
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
American Journal of Pharmacology and Pharmacotherapeutics
ISSN: 2393-8862
W
e have set up an
ex vivo
ovine abomasal model, which can mimic the multicellular process to explore the early steps
in haemonchine nematode infection using RNA-seq technology. Ovine abomasal explants were collected for histological
and transcriptional analysis, supernatants collected to quantitate lactate dehydrogenase (LDH) enzymes. A total of 233 were
substantially induced genes between L4-inoculated and uninoculated-control tissues, respectively. However, a total of 14
were considerably down-regulated genes between the 51 aforementioned tissues. Fifteen pathways were annotated by Kyoto
Encyclopedia of Genes, and Genomes pathway analysis accounted for the significant percentage in immediate response to
larval-stage of
H. contortus
. Key genes up-regulated in response to the addition of L4-inoculum of
H. contortus
were IL-6, IL-8,
C1q, atypical chemokine receptor-3, chemokine ligand-2, manganese superoxide dismutase, integrin alpha-7, -8, -9 , integrin
subunit beta-1, integrin subunit beta 6, intercellular adhesion molecule-1 and actin alpha-1. This study shows for the first time
that galectin-1 is up-regulated in an
ex-vivo
abomasal segment model exposed to L4-inoculum of
H. contortus
following 6 h of
incubation. The abomasal segment model has been shown to be a suitable tool to study the haemonchine larval-stage effects on
the ovine abomasal tissues prior to
in vivo
assessment
.
saeed_elashram@yahoo.comAn ex vivo abomasal ovine model to study the
immediate immune response in the context of
Haemonchus contortus larval-stage
Saeed El-Ashram
Foshan University, Guangdong province, China
Am J Pharmacol Pharmacother 2018, Volume 5
DOI: 10.21767/2393-8862-C1-003