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Pharma 2018
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E u r o p e a n C o n g r e s s o n
Pharma
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
American Journal of Pharmacology and Pharmacotherapeutics
ISSN: 2393-8862
T
he aim of the present investigation was to enhanced topical administration of astaxanthin from activated nanogel. The
solubility study of drug in different surfactant solution was carried out by optimizing the different surfactant concentration at
which maximum drug gets solubilized. Drug-excipients incompatibility study was carried out using Fourier Transforms Infrared
spectroscopy (FTIR). A nanogel based on co-polymerized N-isopropylacrylamide (NIPAM) and butylacrylate (BA) was synthesized,
characterized and loaded with astaxanthin by using emulsion polymerization method. Activated nanogel were evaluated for
organoleptic characteristic, morphological characteristics, gelling property, particle size, zeta potential, percent drug entrapment,
swelling ratio, viscosity, thermal analysis (differential scanning calorimetry), transmission electron microscopy (TEM),
in vitro
drug
permeation on rat skin using franz diffusion cell, skin irritation on rat skin and stability. Fourier Transform Infrared Spectroscopy
(FTIR) study shows that neither drug decomposition nor drug-excipients and excipient-excipient interactions occurred in
the formulation. Solubility of drug was found to be maximum in 1.5% w/v concentration of sodium lauryl sulphate solution.
Activated nanogel shows good organoleptic properties. Transmission electron microscopy confirms the nanogel particles were
monodisperse by having uniform size and spherical shape. The image also serves to validate the purification step, by the absence
of extraneous particulates. Particle size, zeta potential, percent drug entrapment, gelling capacity, viscosity and swelling ratio
was found to be 464.90±2.02 nm, -31.7±2.66 mV, 97.19±0.02%, good, 16,000±707 cps and 13.88±0.16 respectively. Differential
scanning calorimetry indicated that the lower critical solution temperature for poly (N-isopropylacrylamide-co-Butylacrylate) In
deionized water was found to be 31.1˚C and it produced temperature sensitive property.
In vitro
permeation of optimized batch
on rat epidermal membrane using in Franz diffusion cells, followed by the addition of saturated aqueous sodium carbonate
demonstrated the swelling over the range 25-37˚C, provided a astaxanthin flux of 1.69±0.03 µgcm-
2
h
-1
which increased to
0.20±0.0015 µgcm-
2
h
-1
upon the addition of saturated aqueous sodium carbonate up to 24 hrs which suggested that the novel
mechanism is proposed whereby the change in temperature experienced by the nanogel as it penetrated skin induced de-swelling
and expulsion of astaxanthin
in situ. In vitro
skin irritation study indicated that no irritation on rat skin. Stability study indicates
the developed nanogel was stable at 4-8±2°C / 45±5% RH (Refrigerated) condition after 1 month. In the conclusion, activated
nanogel provide nanosized particle size with good percent drug entrapment, increasing flux through skin and better swelling ratio
of polymer could be helpful for the topical administration of astaxanthin with enhanced properly in rheumatoid arthritis condition
.
pateldeepa18@yahoo.comEnhanced topical administration of astaxanthin
from activated nanogel
Deepa H Patel and Rohit V Madariya
Parul Institute of Pharmacy and Research, Parul University, Vadodara, India
Am J Pharmacol Pharmacother 2018, Volume 5
DOI: 10.21767/2393-8862-C1-003