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Pharma 2018
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E u r o p e a n C o n g r e s s o n
Pharma
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
American Journal of Pharmacology and Pharmacotherapeutics
ISSN: 2393-8862
D
iindolylmethane (DIM) is a bioactive metabolite of indole-3-carbinol found in cruciferous vegetables and has anticancer
potential. Stability and sensitivity to the environment are the major challenges for the application of this compound. The
objective of this study was to develop whey protein microencapsulated DIM using the combined heating-ultrasound method.
Solutions with different ratios of DIM to whey protein (1:12, 1:6, 1:4, 1:3, w/w) with constant whey protein (12%, w/v) were heated
at 85˚C for 30 min and then treated with ultrasound for 5, 15, and 30 min, respectively. Zeta potential, particle size, and rheological
property of the samples were studied. Samples after ultrasound treatment showed significantly the reduced particle size of 280-
450 nm and narrowed size distribution (polydispersity index of ~0.47) compared with heated samples (P<0.05). A significant
decrease in zeta potential (P<0.05) was seen when the heated samples (-28.54±54 mV for 1:4 samples) were ultrasound treated
for 5 minutes (-37.667±0.77 mV), 15 min (-33.36±0.85 mV) and 30 min (-31.13±1.02 mV). The viscosity of the ultrasound treated
samples was significantly (P<0.05) decreased as compared to untreated samples. All samples exhibited shear thinning behavior
(pseudoplastic, n<1) and fitted with Sisko model (R
2
>0.997). Consistency index (K
s
) of the samples was increased by ultrasound
treatment. Results indicated that whey protein-based nanoparticles can be used to protect 3, 3′-diindolylmethane for food and
pharmaceutical applications
.
abbaskhan9916@mails.jlu.edu.cnPhysicochemical and rheological properties
of whey protein microencapsulated 3,
3'-diindolylmethane
A Khan
1
, C N Wang
2
, L Li
2
, A Killpartrick
3
, A Humphrey
3
and
M R Guo
2
1
Jilin University, Changchun, China
2
The University of Vermont, Burlington, Vermont, USA
3
FoodScience Corporation, Williston, Vermont, USA
Am J Pharmacol Pharmacother 2018, Volume 5
DOI: 10.21767/2393-8862-C1-003