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Pharma 2018
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E u r o p e a n C o n g r e s s o n
Pharma
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
American Journal of Pharmacology and Pharmacotherapeutics
ISSN: 2393-8862
T
he experimental design was applied for studying and optimizing the different variables affecting the native fluorescence
intensity of moxifloxacin using spectrofluorimetric method. The method was divided into two phases. The first phase is
a pilot stage; a full factorial design was used in order to screen four independent factors and the interaction between them:
temperature (°C) X1, degassing time using the ultrasonicator (min) X2, pH X3 and phosphate buffer concentration (mM) X4.
And the interaction between ((X1, X2), (X1, X3), (X1, X4), (X2, X3), (X2, X4), and (X3, X4)). From the four factors only temperature
(°C) and pH were identified as significant using analysis of variance. The aim of the second phase is to optimize the method’s
performance using central composite face-centered design (CCF). It was found that the optimum conditions of temperature and
pH were 7.4°C and 9.7, respectively. Linearity was observed over the range of 5-40 ng/mL and the detection limit was 0.9 ng/mL.
The optimized method was successfully validated according to the International Conference on Harmonization (ICH) guidelines.
Placket-Burman design was used for method robustness. The method was successfully implemented for the determination of
the commercial tablets with a recovery of 99.64% and a relative standard deviation of 1.33 %.
nohaibrahim2010@gmail.com Noha.ibrahim@miuegypt.edu.egApplying green analytical chemistry for development of a
validated spectrofluorimetric method for determination of
moxifloxacin using the experimental design approach for
screening and optimizing factors affecting its native fluorescence
Noha Ibrahim Shaaban
1
, Said A Hassan
2
, Ahmed E El Gindy
1
and Eman S Elzanfaly
2
1
Misr International University, Cairo, Egypt
2
Cairo University, Cairo, Egypt.
Noha Ibrahim Shaaban et al., Am J Pharmacol Pharmacother 2018, Volume 5
DOI: 10.21767/2393-8862-C1-003