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Volume 3, Issue 2
Insights in Analytical Electrochemistry
ISSN: 2470-9867
Analytical Chemistry-Formulation 2017
August 28-30, 2017
Page 22
8
th
Annual Congress on
&
14
th
International Conference and Exhibition on
August 28-30, 2017 Brussels, Belgium
Analytical and Bioanalytical Techniques
Pharmaceutical Formulations
Development and bioapplications of nontoxic carbon dots
Roger M Leblanc
University of Miami, USA
C
arbon dots (C-Dots) with diameter smaller than 10 nm have recently attracted enormous attention in various fields due
to their unique properties. In this talk, the synthesis, characterization and bioapplications of a new type of nontoxic,
water-soluble C-Dots will be presented. A major medical challenge one faces to treat Central Nervous System (CNS) related
diseases is to cross the tight junctions between endothelial cells, which are known as blood-brain barrier (BBB). Recently, our
in vivo
experimental observations suggested that the transferrin conjugated C-Dots could enter the CNS of Zebrafish while
C-Dots alone could not. Thanks to the abundant presence of carboxylic acids on the surface, CDots are easily conjugated
with transferrin and anticancer drug doxorubicin. The system was then applied as a drug delivery system for the delivery of
doxorubicin into cancerous cells. Our
in vitro
study showed greater uptake of the conjugates compared to free doxorubicin, the
conjugates at 10 nM was significantly more cytotoxic than doxorubicin alone, reducing viability by 14-45 %, across multiple
pediatric brain tumor cell lines. Accidents, disease and aging compromise the structural and physiological functions of bones,
and
in vivo
bone imaging test is critical to identify, detect and diagnose bone related development and dysfunctions. Here we
show that C-Dots with low quantum yield ("dark") bind to calcified bone structures of live Zebrafish larvae with high affinity
and selectivity. Binding resulted in a strong enhancement of luminescence that was not observed in other tissues, including
non-calcified endochondral elements. Retention of Cdots by bones was very stable, long lasting, and with no detectable
toxicity. These observations support a novel and revolutionary use of C-Dots as highly specific bioagents for bone imaging and
diagnosis, and as a potential bone-specific drug delivery carrier.
Biography
Roger M Leblanc received his BS in Chemistry in 1964 from Universite Laval, Canada, and PhD in Physical Chemistry in l968 from the same university. From 1968
to 1970, he was a Postdoctoral Fellow in the Laboratory of Prof. George Porter, FRS, in Davy Faraday Research Lab, the Royal Institution of Great Britain. He
was a Professor from 1970 to 1993 at Department of Chemistry and Biology in Universite du Quebec a Trois Rivieres, Canada. During this period, he was Chair
from 1971 to 1975 at the same department, and Director from 1981 to 1991 at Photobiophysics Research Center. In 1994, he moved to University of Miami, where
he has been a Professor at Department of Chemistry since then to present. At University of Miami, he was Chair of Department of Chemistry from 1994 to 2002,
and he is appointed as Chair from 2013 to present. He was also one of the three editors of
Colloids and Surfaces B: Biointerfaces
from 1998 to 2017. During his
early career as a Scientist, his research interest was on the photosynthesis and photoconductivity using surface chemistry and spectroscopy. His current research
interest is to apply 2-dimensional (2-D) surface chemistry combined with spectroscopy and microscopy to investigate the properties of nanomaterials (carbon dots,
graphene oxide and quantum dots) and the fibrillation process of amyloidogenic proteins (insulin, amyloid-beta peptide and islet amyloid polypeptide). He is also
interested to design and develop biosensors with high sensitivity and selectivity for diseases diagnosis. He has published 512 scientific articles in peer-reviewed
journals. As a Professor, he has supervised more than 100 Master’s and PhD students.
rml@miami.eduRoger M Leblanc, Insights in Analytical Electrochemistry, 3:2
DOI: 10.21767/2470-9867-C1-002