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Volume 3, Issue 2 Insights in Analytical Electrochemistry
ISSN: 2470-9867 Analytical Chemistry-Formulation 2017
August 28-30, 2017
Page 18
8 th Annual Congress on
&
14th International Conference and Exhibition on
August 28-30, 2017 Brussels, Belgium
Analytical and Bioanalytical Techniques
Pharmaceutical Formulations
A potential universal cancer biomarker revealed by bioimaging of fluorescent probes for point-of-
care screening of cancer
Ta-Chau Chang1,2, Ting-Yuan Tseng1, Wei-Wen Chen1
1Academia Sinica, Taiwan
2National Taiwan University, Taiwan
C ancer remains as one of the leading causes of death in many countries. Cancer is not a single disease but a complex
progression of cellular/tissue mutation. Currently, no “universal” cancer biomarker has been documented. It is a great
challenge to find a universal cancer biomarker. Finding a common target of intracellular difference between cancer and normal
cells is extremely important for cancer prevention, detection, and treatment. A single-stranded Guanine-rich (G-rich) sequence
is capable of forming G-quadruplex (G4) via Hoogsteen hydrogen bonds under physiological condition. G4 oligonucleotides
have recently gained much attention as a possible target for cancer research. Fluorescent probe together with optical imaging
provides a means of visualizing the possible differences between cancer cells and normal cells. A fluorescent probe, 3,6-bis(1-
methyl-2-vinylpyridinium) carbazole diiodide (o-BMVC), showed a large contrast in binding affinity to DNA of ~107 for G4s
and ~105 for duplexes. Moreover, the fluorescent decay time of o-BMVC is longer (≥2.4 ns) upon binding to most G4s such as
G-rich sequences in telomeres and some promoter oncogenes, while the decay time is shorter (~1.2 ns) upon interaction with
duplex structures such as linear duplexes
Biography
Ta-Chau Chang was awarded his PhD degree from Iowa State University, USA 1985. He was a visiting fellow in CIRES at Boulder for one year and a postdoctoral
fellow at University of Illinois at Urbana for two years. He went back to Taiwan and joined the Faculty of Institute of Atomic and Molecular Sciences, Academia
Sinica 1988. His current research interests focus on the development and application of fluorescent theranostic agents in cancer research and advanced optical
methods, and G-quadruplex in biomedical research.
Ta-Chau Chang et al., Insights in Analytical Electrochemistry, 3:2
DOI: 10.21767/2470-9867-C1-002