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Volume 3, Issue 2
Insights in Analytical Electrochemistry
ISSN: 2470-9867
Analytical Chemistry-Formulation 2017
August 28-30, 2017
Page 17
8
th
Annual Congress on
&
14
th
International Conference and Exhibition on
August 28-30, 2017 Brussels, Belgium
Analytical and Bioanalytical Techniques
Pharmaceutical Formulations
Determination of capecitabine and its metabolites in plasma of Egyptian colorectal cancer patients
Rasha Hanafi
1
S Shams
1
, S Abdel-Maksoud
1
, S Eid
2
and
M Gad
1
1
German University in Cairo, Egypt
2
National Cancer Institute, Egypt
C
olorectal Cancer (CRC) is constantly increasing in incidence both worldwide and at the national level. Chemotherapeutic
agents often prescribed in CRC are Capecitabine (CCB) and 5-Fluorouracil (FU). CCB is activated to FU in a three steps
reaction giving 5'-deoxy-5-fluorocytidine (DFCR), followed by 5'-deoxy-5-fluorouridine (DFUR) to yield finally FU, the active
form, which is later deactivated to 5,6-dihydro-5-fluorouracil (DHFU). Patients exhibited variable responses and adverse events
in response to CCB therapy, despite being treated with the same dose. This could be explained by the presence of different
possible enzyme SNPs that can occur along the CCB activation and deactivation pathways. This study aims at developing a
new method of analysis of CCB and its metabolites using HPLC-UV, to determine the plasma concentrations of CCB and its
metabolites DFCR, DFUR, FU, DHFU and 5-Chlorouracil (CLU; the internal standard), followed by a correlation study with
the toxicities occurring during therapy, to become a predictive method for toxicity, away from the exhausting genotyping
process. A new superior analytical method is presented using computer-assisted method development, which achieved full
separation of the six analytes during the least possible gradient time, eluting the compounds at 2.8, 3.2, 4.4, 5.2, 5.8 and 9.9
minutes for DHFU, FU, CLU, DFCR, DFUR and CCB, respectively. The method showed accuracy, precision and robustness
upon validation. Clinical results showed a positive correlation between the DFCR concentration and mucositis, as well as,
between the DFUR concentration and Hand-Foot Syndrome, confirming that this technique could be used for predicting such
toxicities in CRC patients.
Biography
Rasha Hanafi joined the faculty of Pharmacy, Department of Pharmaceutical Chemistry in 2004, where she is currently Associate Professor of Instrumental
Analysis and Analytical Chemistry. Dr. Hanafi received her pharmacy bachelor and her master’s degree in pharmaceutical analysis from the faculty of Pharmacy,
Cairo University in 1996 and 2005, respectively, and her PhD. degree from the faculty of Pharmacy, German University in Cairo in 2009 in biomedical analysis.
She was promoted to associate professor in the Supreme Council of Universities in 2016. She is reviewer of international journals, has large number publications
in peer reviewed journals and has presented in many conferences around the world. She supervised a large number of PhD and master students in the field of
pharmaceutical and biomedical analysis. Dr. Hanafi’s research involves analytical method development and validation within the Quality by Design framework, and
is is a consultant and trainer for industry and academia.
rasha.hanafi@guc.edu.egRasha Hanafi et al., Insights in Analytical Electrochemistry, 3:2
DOI: 10.21767/2470-9867-C1-002