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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 38
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
E
nhancement of cholinergic function by inhibition of
acetylcholinesterase (AChE) is considered as a rational
approach for the treatment of neurological disorders such as
Alzheimer´s disease and senile dementia. During the last two
decades, the use of herbal medicinal substances in dementia
therapy has been studied well. Iran is among those countries
which has a long and rich history in traditional medicine. In
this eight year project, 40 herbal drugs reported in Iranian
traditional medicine (ITM) for the treatment of cognitive
disorders were examined. In the first step, the bioactivities of
the polar methanol and the non-polar dichloromethane extracts
of all selected herbal samples were studied on AChE inhibition
by TLC bioautography and in a microplate assay. The 8 most
active herbal drugs were selected for further study. In detailed
investigations, the active compounds were identified and
isolated using several chromatographic techniques such as
TLC, VLC, LC, SEC, SPE, HPLC, HPCCC and HPTLC. In total, from
the selected herbal drugs, 25 active compounds were identified
and isolated. The structures of the active components were
characterized by one and two-dimensional NMR spectroscopy
(COSY, TOCSY, HSQC, HMBC, NOESY), mass spectrometry and
some other analytical methods. The IC50 values for active
compounds were determined by a quantitative colorimetric
assay. Additionally, the concentrations of active components
in their sources were determined by HPLC analysis. The IC50
values of the isolated compounds were in correlation with
their contents in their original sources. The achieved results
confirmed that the compounds considerably contribute to the
effects of these drugs and underline the plausibility of their use
in the treatment of cognitive deficits in ITM.
Recent Publications
1. Kanama S K, Viljoen A M, Kamatou G P P, Chen W,
Sandasi M, et al. (2015) Simultaneous quantification
of anthrones and chromones in
Aloe ferox
(“Cape
aloes”) using UHPLC–MS. Phytochemistry Letters
13:85–90.
2. Adhami H R, Zehl M, Dangl C, Dorfmeister D, Stadler
M, et al. (2015) Preparative isolation of major phenolic
compounds from extra virgin olive oil by HPCCC. Food
Chemistry 170:154–159.
3. Adhami H R and Viljoen A M (2015) Isolation of
bio-markers from the leaf exudate of
Aloe ferox
(aloe bitters) by high performance counter-current
chromatography. Phytochemistry Letters 11:321–325.
4. Adhami H R, Fitz V, Lubich A, Kaehlig H, Zehl M,
et al. (2014) Acetylcholinesterase inhibitors from
galbanum, the oleo gum-resin of
Ferula gummosa
Boiss. Phytochemistry Letters 10:32–87.
5. Adhami H R, Lutz J, Kaehlig H, Zehl M and Krenn L
(2013) Compounds from gum ammoniacum with
acetylcholinesterase inhibitory activity. Scientia
Pharmaceutica 81(3):793–805.
Biography
Hamid-Reza Adhami graduated from the Faculty of Pharmacy, Tehran University
of Medical Sciences with a PharmD Degree and worked for some years in the field
of Health and Research Management. In 2008, he started his PhD study at the
Department of Pharmacognosy, University of Vienna. After graduation, he passed
short term research projects in Switzerland and UK and finally moved to South
Africa for a one year Post-Doc Fellowship at Tshwane University of Technology in
Pretoria. Since 2014, he is working as Assistant Professor at the Department of
Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences. His
research interests include isolation, characterization and purification of biological
active secondary metabolites from natural sources.
hr-adhami@tums.ac.irKnowledge of traditional medicine toward enhancement of
cognitive performance
Hamid-Reza Adhami
Tehran University of Medical Sciences, Iran
Hamid-Reza Adhami, Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-005