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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 36
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Statement of the Problem:
Skin ageing processes are generally
divided into intrinsic, irremediably tied to the passage of time,
and extrinsic, which are caused by environmental factors (i.e.
chronic exposure to sunlight, pollutants) and miscellaneous
lifestyle components. Plants are a precious resource for
skincare, both as antioxidants and inhibitors of enzymes
involved in the ageing process. In this context, elastase and
tyrosinase are target of remarkable importance, and their
inhibitors find applications as skin whitening, anti-wrinkle,
anti-sagging agents, and in the treatment of dermatological
disorders.
Methodology:
The plant sources were from: India (Rajasthan),
Africa (Burkina Faso) and Mediterranean area. Hydroalcoholic
extracts were prepared, dried and dissolved in water for the
assays or in water-d2 phosphate buffer (90 mM; pH 6.0) for
1H–NMR analysis.
Findings:
17 plants resulted to be endowed with strong
bioactivity and leaves of Pistacia lentiscus emerged as the
most potent sample on both enzymes (IC50 of 7.18 ±1.37
and 42.04±1.94 μg/mL against elastase and tyrosinase,
respectively). Interestingly, two, out of the 17most active plants,
are endemic of Sardinia Island (Italy), namely: Hypericum
scruglii and Limonium morisianum. Moreover, the 17 most
active extracts resulted also generally enriched in polyphenols
and flavonoids, which confer them additional value, due to their
antioxidant potential. Lastly, 1H NMR metabolomics profile
of the extracts were measured, compared by multivariate
data analysis and correlated to the abovementioned results
by orthogonal projections to latent structures (OPLS) model,
showing a positive correlation between spectral signal of
aromatic compounds and the potency of enzymatic inhibition.
Conclusion & Significance:
This bio-screening allowed
the selection of 17 plant extracts, promising as cosmetic
ingredients, which safety is also supported by the
ethnobotanical uses. Nine of them proved active both against
tyrosinase and elastase and as shown by the OPLS model their
aromatic phytoconstituents play a key role in conferring these
bioactivities.
Recent Publications
1. Liyanaarachchi G D, Samarasekera J K, Mahanama
K R and Hemalal K D (2018) Tyrosinase, elastase,
hyaluronidase, inhibitory and antioxidant activity of
Sri Lankan medicinal plants for novel cosmeceuticals.
Industrial Crops and Products 111:597–605.
2. Pillaiyar T, Manickam M and Namasivayam V
(2017) Skin whitening agents: medicinal chemistry
perspective of tyrosinase inhibitors. Journal of Enzyme
Inhibition and Medicinal Chemistry 32:403–25.
3. Imokawa G and Ishida K (2015) Biological mechanisms
underlying the ultraviolet radiation-induced formation
of skin wrinkling and sagging I: reduced skin elasticity,
highly associated with enhanced dermal elastase
activity, triggers wrinkling and sagging. International
Journal of Molecular Sciences 16:7753–7775.
4. Mukherjee P K, Maity N, Nema N K and Sarkar B K
(2011) Bioactive compounds from natural resources
against skin aging. Phytomedicine 19:64–73.
5. Yuliana N D, Khatib A, Choi Y H and Verpoorte R (2011)
Metabolomics for bioactivity assessment of natural
products. Phytotherapy Research 25:157–69.
Screening of 100 plant extracts as tyrosinase and elastase
inhibitors, two enzymatic target of cosmetic interest
Manuela Mandrone
University of Bologna, Italy
Manuela Mandrone, Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-005