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Medchem & Toxicology 2018
Page 87
Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123
A n n u a l C o n g r e s s o n
Medicinal Chemistry,
Pharmacology and toxicology
J u l y 3 0 - 3 1 , 2 0 1 8
Am s t e r d a m , N e t h e r l a n d s
H
elicobacter pylori
chronic infection is associated, among other severe gastric disorders, with intestinal-type gastric
carcinogenesis, being this the fifth most common cancer and the third leading cause of cancer-related death worldwide.
Classical
H. pylori
eradication treatment, combining two antibiotics and a proton pump inhibitor, reduces the risk for gastric
carcinoma development, but treatment of
H. pylori
infection is challenged by a dramatic fall in eradication rates all over the world.
Currently, this bacterium is listed among the 16 antibiotic-resistant bacteria that pose greatest threat to human health according
to the World Health Organization. Antimicrobial peptides (AMPs) present an alternative to conventional antibiotic therapies,
being their most striking feature the low tendency to induce bacterial resistance, since AMPs selectively damage the bacterial
membranes through mechanisms that bacteria find difficult to evade. In an
in vivo
scenario, “unbound AMPs” can undergo
proteolysis and peptide aggregation, leading to efficiency decrease. AMP grafting onto nanoparticles has been reported as a
good strategy to protect peptides from aggregation and enzymatic degradation in vivo, therefore increasing long-term stability
and avoiding cytotoxicity is associated with application of high AMP concentrations. In this study, we demonstrated that the
AMP MSI-78A could be surface-grafted without compromising its activity. Moreover, MSI-78A-decorated surfaces were highly
effective against
H. pylori
, killing bacteria by contact in a short time span, since after 2 hrs only, 2% of
H. pylori
remained viable in
suspension. These results encourage the utilization of grafted MSI-78A on biocompatible nanoparticles as an alternative to the
currently available therapy against H. pylori, opening new routes for gastric infection management
.
parreira@i3s.up.ptManagement of H. pylori gastric infection via
surface-grafted antimicrobial peptides
Paula Parreira1,2, Claudia Monteiro1,2, Vanessa Graça2, Joana
Gomes1,3, Sílvia Maia4, Paula Gomes4, Inês C Gonçalves1,2,5
and M Cristina L Martins1,2,6
1
i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal
2
INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Portugal
3
IPATIMUP, Instituto de Patologia e Imunologia Molecular, Universidade do Porto, Portugal
4
LAQV-REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade
do Porto, Portugal
5
FEUP, Faculdade de Engenharia, Universidade do Porto, Portugal
6
Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Portugal
J Org Inorg Chem 2018, Volume 4
DOI: 10.21767/2472-1123-C3-009