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Medchem & Toxicology 2018

Page 92

Journal of Organic & Inorganic Chemistry

ISSN: 2472-1123

A n n u a l C o n g r e s s o n

Medicinal Chemistry,

Pharmacology and toxicology

J u l y 3 0 - 3 1 , 2 0 1 8

Am s t e r d a m , N e t h e r l a n d s

Background:

Hot pepper (

Capsicum species

) is an economically important spice widely cultivated and consumed in Ethiopia.

In spite of its wide importance, there is no information available on molecular genetic diversity of this crop. The importance

of cultivars characterization is an important link between the conservation and utilization of plant genetic resources in various

breeding programmes

Result:

Using five ISSR primers, a total of 37 scorable bands were generated of which 35 (94.6%) were polymorphic. Within

population diversity based on polymorphic bands ranged from 51.35% to 91.89 % with a mean of 66.6 %, Nei’s genetic diversity

of 0.19-0.30 with a mean of 0.28, and Shannon information index of 0.29-0.45 with a mean of 0.43. With all diversity parameters,

the highest diversity was obtained from Amhara2 populations, whilst the lowest was from Oromia2. From Jaccard’s pairwise

similarity coefficient, Oromia1 and Oromia2 were most related populations exhibiting 0.956 similarity and Semn omo and Amhara

2 were the most distantly related populations with similarity of 0.827. Clustering was showed that there is strong correlation

between geographic distance and genetic diversity of Ethiopian hot peppers cultivars because geographically closely related

species have been clustered together.

Conclusion:

Amhara 2 populations (from West Gojjam and North Gonder) exhibited the highest genetic diversity so that the

populations should be considered as the primary sites in designing conservation areas for this crop. Further, it is suggested that

molecular markers are valid tags for the assessment of genetic diversity in

Capsicum species

cultivars.

shifeey@gmail.com

Genetic diversity study of Ethiopian hot pepper

cultivars (Capsicum species) using Inter Simple

Sequence Repeat (ISSR) Marker

Shiferaw Alemu, Destaw Mulaleam, Samuel Adugna

Wolkite University, College of Natural and Computational Sciences, Ethiopia

J Org Inorg Chem 2018, Volume 4

DOI: 10.21767/2472-1123-C3-009