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August 17-18, 2017 | Toronto, Canada
ANNUAL BIOTECHNOLOGY CONGRESS
Ann Biol Sci, 2017
ISSN: 2348-1927
T
he major application of Biotechnology is the transfer of
desirable characteristic in the host. To attain resistance
against insects and weeds, this application was employed
to transfer a double Bt and a glyphosate gene into two
cotton varieties, FH-114 and CIM-598. Three genes Cry1Ac,
Cry2A and Glyphosate gene were transferred through the
Agrobacterium method using a plant expression vector with
genes under the control of the CaMV35S promoter and
NOS terminator sequence. Confirmation of insertion and
expression of these genes in cotton plants was done through
PCR and ELISA. Transformation efficiency for FH-114 and
CIM-598 was 1.2% and 0.8% for Cry1Ac, 0.9% and 0.6% for
Cry2A and 1.5 and 0.7% for GTG respectively. FH-114 plants
acclimatized better than CIM-598 plants when exposed to
sunlight. Cry1Ac, Cry2A and GTG proteins were 1.2, 1 and
1.3 ngµl-1 for FH-114 which was more than CIM-598 for
all three genes. FH-114 plants were able to control better
insects and weed damage when subjected to a cotton leaf
bioassay. Taken together, FH-114 genetic profile was more
suitable for genetic modification to control insects and weed
when compared to CIM-598.
e:
Tahir_samiullah@yahoo.comAnalysis of genetically modified BT and cp4EPSPS cotton cultivars for transformation efficiency,
acclimatization, expression and toxic levels to insects
Tahir Rehman Samiulla
University of the Punjab, Pakistan
Ann Biol Sci, 2017, 5:3
DOI: 10.21767/2348-1927-C1-003