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August 17-18, 2017 | Toronto, Canada
ANNUAL BIOTECHNOLOGY CONGRESS
Ann Biol Sci, 2017
ISSN: 2348-1927
G
lyphosate weed control is a very effective strategy to
minimize cost and improve economic outcomes of
world and Pakistan agriculture production. Development of
glyphosate-resistant potato hold great promise. A new G2-
aroA gene from Pseudomonas florescence which encodes
5-enolpyruvylshikimate-3-phosphate
synthase
(EPSPS)
was transformed by using an Agrobacterium-mediated
transformation into potato cultivate AGB-Red. Transgenic
potato plants were generated vis node tissue culturemethod,
using kanamycin selection. Ten regenerated potato plants
were obtained and allowed to grow normally in pots under
normal conditions. The Polymerase Chain Reaction (PCR),
Southern Blotting and Western Blotting analysis confirmed
that the target gene was integrated and expressed effectively
into potato chromosomes at the very potential level. The
glyphosate tolerance assay showed that transgenic potato
had a high resistance level to glyphosate. Furthermore,
potato plants treated with 50.0 mmolL of glyphosate could
grow slowly and can develop tubers. It was concluded that
transgenic potato may be used for cotton breeding research
of glyphosate-tolerant potato.
e:
arfan.ali@cemb.edu.pkDevelopment of glyphosate-tolerant transgenic potato plants harboring the G2-aroA gene
Arfan Ali
1, 2
1
Fb Genetics Limited, Lahore, Pakistan
2
University of the Punjab, Pakistan
Ann Biol Sci, 2017, 5:3
DOI: 10.21767/2348-1927-C1-003