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Page 56

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August 17-18, 2017 | Toronto, Canada

ANNUAL BIOTECHNOLOGY CONGRESS

Ann Biol Sci, 2017

ISSN: 2348-1927

T

he abuse of antibiotics has raised the prevalence of

antibiotic resistance, and the high frequency of antibiotic

resistance will be a serious global health concern. Landfill

is the primary treatment for municipal solid waste, and the

generated leachate will be the important hotspot of the

antibiotics and antibiotic resistant genes (ARGs). Until now,

no effective on-site treatment has been put forward for

preventing ARGs dissemination during leachate treatment.

Herein, the aged refuse bioreactor was employed to

remove antibiotics and ARGs from leachate, and the great

removal performance was observed. For the detected

antibiotics, the total removal efficiency was about 76.75%,

and sulfanilamide and macrolide were removed with high

efficiencies (>80%). Among the target ARGs, tetracycline

and macrolide resistance genes (

tetM, tetQ and ermB

)

were eliminated with 1.2-2.0 orders of magnitude. The

occurrences of ARGs did not correlate with physicochemical

parameters, but closely linked to the variations of the

bacterial community structure. Redundancy analysis (RDA)

indicated the significant correlations between four genera

and the distribution of ARGs, which implied that these key

genera (including potential pathogens) drove the ARGs

removal. Furthermore, the hydraulic loading test confirmed

that the aged refuse bioreactor was capable of achieving

high removal efficiencies even under shock loading and for

the higher loading it was negative for the proliferations of

potential ARGs hosts. This study suggested that aged refuse

bioreactor could be a promising way for antibiotics and ARGs

on-site removal from leachate.

e:

bxie@des.ecnu.edu.cn

On-site removal of antibiotics and antibiotic resistant genes from leachate by aged refuse bioreactor:

effects of microbial community and operational parameters

Bing Xie and Yinglong Su

East China Normal University, China

Ann Biol Sci, 2017, 5:3

DOI: 10.21767/2348-1927-C1-003