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Archives of Clinical Microbiology
ISSN: 1989-8436
Virology and Infectious Diseases 2019
EuroSciCon Conference on
Virology and Infectious Diseases
O
ncolytic viral therapies against cancers, using variously attenuated or recombinant viruses, have appeared as
a promising method in cancer treatment in recent years. Orf virus (ORFV) strain NZ2 has been shown to have
antitumor effects in animal models mediated by immunoregulation profile, however, little is known about the molecular
cellular mechanism of orf virus’s anti-cancer effect. Here we report ORFV strain NA1/11, isolated from a sheep in Jilin
province of China, inhibited the growth of colorectal cancer (CRC) cells lines including Caco-2, HCT116, LoVo, RKO,
SW480, SW1116 cells. ORFV strain NA1/11 also significantly inhibited the growth and the pulmonary metastasis of CRC
cells
in vivo
. The inhibitory mechanism of ORFV strain NA1/11 involved apoptosis and autophagy induction. Besides,
we utilized a cytokine antibody array to develop a more comprehensive description of the cytokines by ORFV, which
indicated that ORFV likely plays roles in the regulation of key factors relevant to apoptosis, autoimmunity/inflammation,
angiogenesis and the cell cycle for further molecular mechanism studies. These results suggested that ORFV could be
an oncolytic virus for CRC therapy.
sluo815@gmail.comIdentification of oncolysis effect in colorectal
cancer cells by Orf virus strain NA1/11
in vitro
and
in vivo
Shuhong Luo and Ruixue Wang
Foshan University, China
Arch Clin Microbiol 2019, Volume:10
DOI: 10.4172/1989-8436-C1-018