21 / 27
A p r i l 2 2 - 2 3 , 2 0 1 9
A t h e n s , G r e e c e
Page 73
Archives of Clinical Microbiology
ISSN: 1989-8436
Virology and Infectious Diseases 2019
EuroSciCon Conference on
Virology and Infectious Diseases
M
arine ecosystems represent a promising source of organisms with a wide spectrum of biomedical applications.
The brown seaweeds of Sargassum genus embrace a varied biochemical composition and synthesize metabolites
with antiviral activities. Becoming then in potential candidates for searching and developing drugs against viral diseases
that show drug resistance or lack of therapy. The goal of this work is to evaluate and characterize the antiviral activity
of different extracts from seaweeds of the Caribbean against reference and clinical strains of some human enterovirus
and respiratory virus. The qualitative determination of secondary metabolites (phytochemical identification) from the
extracts was made according to Rondina and Coussio, 1969. The cytotoxicity of the extract was evaluated in Vero, RD
and Hep-2 cells by MTT method and the CC50 was calculated by lineal regression analysis. Determination of antiviral
activity (EC50) was made by means of cytopathic effect inhibition in cells. The extracellular virucidal activity of the
extract and the reduction of viral yield were determined by viral titration through final dilution assay. The antiviral activity
was characterized by an addition of time assay. The preliminary characterization of the extract showed compounds
such as tannins, quinones, proanthocyanidins/catechins, triterpens, proteins and polysaccharides. The extract was
non-cytotoxic at the evaluated concentrations and showed an inhibitory activity against the viruses. Besides, it showed
virucidal activity and diminished the viral titer more than three log compared to the control. The extract was active
on the early and late stages of viral replication. The presence of actives compounds might be related to the showed
antiviral activity, supporting so the potential of these seaweeds as a source of antivirals.
lponce@fbio.uh.cu lienaponce@gmail.comAntiviral activity of Caribbean seaweeds against
human virus with clinical and epidemiological
significance
Liena de Regla Ponce Rey
1
, Del Barrio G
1
, Roque A
1
, Resik S
1
and
Spengler I
2
1
Havana University, Cuba
2
Institute of Tropical Medicine "Pedro Kouri", Cuba
Arch Clin Microbiol 2019, Volume:10
DOI: 10.4172/1989-8436-C1-018