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Archives of Clinical Microbiology
ISSN: 1989-8436
Virology and Infectious Diseases 2019
EuroSciCon Conference on
Virology and Infectious Diseases
Background & Aim:
Bovine tuberculosis is one of the most important zoonotic diseases in Bovidae. Humans and
animals that transit to the farm can transfer
Mycobacterium
to the cattle. Hence, the aim of this study is to evaluate the
possible role of cats in transferring the
Mycobacterium
infection in dairy farms.
Methods:
From a dairy cattle farm with more than 20% infection of
Mycobacterium
, seven cats were captured and their
gastric juice cultured in the LJ and LGmedium. The acid-fast staining of the isolates prepared to identify
Mycobacterium
and PCRs were carried out afterwards.
Results:
Five out of seven cultures were positive in direct smear by acid fast staining and in PCR-16SrRNA, which
indicates that the above-mentioned isolates belong to the
Mycobacterium
genus. Also, positive PCR-IS6110 confirmed
that the isolate species are identified as
Mycobacterium
tuberculosis complex. Currently, we are conducting sequencing
for the exact identification of these isolates.
Conclusion:
Animals such as mice and cats that live in the farm can harbour
Mycobacterium
. In this study, it has been
proven that cats certainly transfer
Mycobacterium
to the cattle farms.
khashaiar.mansouri.dvm@gmail.comIsolation and identification of
Mycobacterium
from captured cats belonging to tuberculosis
infected farms
Khashaiar Mansouri
1
and Nader Mosavari
2
1
Islamic Azad University-Garmsar Branch, Iran
2
Razi Vaccine and Serum Research Institute-AREEO, Iran
Arch Clin Microbiol 2019, Volume:10
DOI: 10.4172/1989-8436-C1-018