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A p r i l 2 2 - 2 3 , 2 0 1 9
A t h e n s , G r e e c e
Page 68
Archives of Clinical Microbiology
ISSN: 1989-8436
Virology and Infectious Diseases 2019
EuroSciCon Conference on
Virology and Infectious Diseases
T
he present study was undertaken in order to investigate the role of gentamicin in the gene expression of toxA in
Pseudomonas aeruginosa
isolated from cow mastitis. A total of ten
P. aeruginosa
strains originally isolated from
cows infectedwithmastitis. Agar dilutionmethodologywas performed to determine theminimal inhibitory concentration
of gentamicin, all of which developed resistance toward gentamicin. The findings presented here demonstrated that all
these strains harboured toxA depending on PCR-based assay. Nonetheless, RT-PCR technique revealed a wide variation
in expression of toxA. Moreover, the cultivation of
P. aeruginosa
in the presence of gentamicin, significantly (P<0.05),
induced the expression of toxA, in addition to the possibility of enhancing the virulence of this bacterium. In conclusion,
using gentamicin to treat infections caused by
P. aeruginosa
may participate in more severe outcomes.
aaldouri96@yahoo.comGentamicin enhances toxA expression in
Pseudomonas aeruginosa
isolated form cow
mastitis
Atheer Abdul Razzaq and Ansam KhAlid Mahmood
University of Baghdad, Iraq
Arch Clin Microbiol 2019, Volume:10
DOI: 10.4172/1989-8436-C1-018