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Pharmacognosy 2019
March 11-12, 2019
London, UK
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 51
Pharmacognosy and
Medicinal Plants
7
th
Edition of International Conference on
Am J Ethnomed 2019, Volume 6
DOI: 10.21767/2348-9502-C1-009
Some biological active phenolic compounds from
Polygonatum obtusifolium
Lili Gvazava
TSMU - Iovel Kutateladze Institute of Pharmacochemistry, Georgia
I
n continuation of the search for biologically active
compounds in plants of the Georgian flora, we
studied the chemical composition of
Polygonatum
L.
(Convallariaceae). Species of the genus
Polygonatum
L.
are spread in Caucasus. In traditional medicine they are
used as a haemostatic, anti-inflammatory and wound
healing activity. Raw materials (air-dried ground roots)
were extracted with MeOH (80%, 3x) for 1h at reflux.
The solvent was distilled off. The remaining aqueous
phase was worked up with hexane to remove lipophilic
substances and then extracted successively with CHCl3,
EtOAc and n-BuOH. After the solvents were removed
and the EtOAc and BuOH extracts that contained three
stilbenes were combined and chromatographed over a
column of sephadex LH-20 with elution by H
2
O:MeOH
with increasing two alcohol content. This produced
eight fractions and the fractions containing stilbenes
were combined and rechromatographed over an
analogous column with elution by EtOH to afford two
pure compounds 1 (0.078 g, 0.019% calculated per raw
material mass) and 2 (0.110 g, 0.027%). Compound
1: C15H14O5, [M]+ 274, oily liquid. IR spectrum (KBr,
vmax, cm-1): 3360 (OH), 3005, 2850, 1580, 1520, 1440
(aromatic), 1150 (C–O), 860, 845 (substituted rings), 680
(cis-bonded CH=CH). A comparison of the spectral data
with the literature identified1ascis-3,5,3’,5’ -tetrahydroxy-
4-methoxystilbene. Compound 2: C14H15O5, [M]+ 274,
colorless crystals, Mp 167–169°C. IR spectrum (KBr,
vmax, cm-1): 3270 (OH), 3005, 2840, 1620, 1595, 1520,
1430 (aromatic), 1160 (C–O), 980 (trans-bondedCH=CH),
835, 675 (substituted rings). Acomparison of the spectral
data with the literature identified 2 as trans-3,5,3’,5’
-tetrahydroxy-4-methoxystilbene. Compound 3: C9H6O3,
[M]+ 162, colorless prisms or needles, Mp 234–235°C.
UV spectrum (EtOH, max, nm): 320, 255. It was identified
as umbelliferone (7-hydroxycoumarin). Compound 4:
C10H8O4, [M]+ 192, colorless or yellowish crystals, Mp
202–204°C. UV spect¬rum (MeOH, max, nm): 233, 255,
295, 347. It was identified as scopoletin (6-methoxy-7-
hydroxy¬cou¬marin). All compounds were isolated for
the first time from
P. obtusifolium
.