pharmacognosy-2019-posters-abstracts

Pharmacognosy 2019

March 11-12, 2019

London, UK

American Journal of Ethnomedicine

ISSN: 2348-9502

Page 55

Pharmacognosy and

Medicinal Plants

Edition of International Conference on

Am J Ethnomed 2019, Volume 6

DOI: 10.21767/2348-9502-C1-009

Mechanisms behind hypoglycemic actions of butanol

fraction of

Azadirachta indica

in a type 2 diabetes rat model

Olakunle Sanni and Md Shahidul Islam

University of Kwazulu-Natal, South Africa

he cost and side effects of oral hypoglycemic agents

for the treatment andmanagement of type 2 diabetes

(T2D) has led to increase the use of natural medicines,

particularly from medicinal plants. Hence the validation

of the folkloric and use of these medicinal plants. In the

present study, the mechanisms behind the antidiabetic

effects of the butanol fraction of

Azardirachta indica

(A. indica)

(BFAI) were evaluated. T2D was induced by

feeding 10% fructose solution ad libitum for two weeks

followed by an intraperitoneal injection of streptozotocin

(40 mg/kg body weight) and the animals were treated

with a low dose (150 mg/kg) and a high dose (300

mg/kg) of BFAI for 4 weeks as a single oral dose daily.

Body weight and blood glucose were determined every

week. Oral glucose tolerance test was performed in the

last week of treatment. Insulin homeostasis and liver

glycogen concentration were determined after 4 weeks

of oral administration. Both doses of the fractions

showed significant improvement of body weight, reduced

blood glucose, stimulated insulin secretions, improved

pancreatic β- cell function (HOMA-β), decreased insulin

resistance (HOMA-IR) and increased liver glycogen

concentration compared to untreated diabetic rats,

when high dose had a better activity. GC-MS analysis of

the fraction revealed the presence of polyphenols. The

results of this study suggest that, the polyphenols present

in the fraction may be responsible for the antidiabetic

effects which have been achieved via decreasing insulin

resistance, modulating β-cell function, as well as by

inhibiting of α-amylase and α-glucosidase activity.