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Pharmacognosy 2019
March 11-12, 2019
London, UK
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 48
Pharmacognosy and
Medicinal Plants
7
th
Edition of International Conference on
Am J Ethnomed 2019, Volume 6
DOI: 10.21767/2348-9502-C1-009
Methanolic extract leaf of
Scabiosa atropurpurea
promote
an anticancer activity
in vitro
and demonstrate significant
impact on tumor growth
in vivo
Leila Chekir Ghedira
Faculty of Dental Medicine of Monastir, Tunisia
S
cabiosa atropurpurea
(S.atrp) was extracted with
methanol then the chemical composition was
identified by HPLC MS/MS (High Performance Liquid
Chromatography Mass spectrometry). The current study
aims to investigate the multidrug resistance (MDR) of
colorectal cancer cells which is amultidimensional problem
involving several mechanisms and targets. This study
demonstrates that Methanolic extract from S.atrop, which
contains flavonoids, phenolic acids and saponins has the
ability to inhibit cell proliferation and reverse resistance of
some colorectal cancer cells (Caco-2, HCT-116 and CT26)
to the cisplatin chemotherapy (CDDP), by induction of
apoptosis. This extract promoted G2/M and S arrest at
48h and it also overcame P- gp/MDR1 and MRP1 activities
in a concentration-dependent. Moreover, it inhibited the
activity of the drug modifying enzyme GST. Besides, this
study evaluated the antitumoral effect of this extract.
BALB/c mice were subcutaneously inoculated with CT-26
cells, then treated intra-peritoneally after 7 days with the
methanolic extract for 21 days. Our results showed that
S.atrop could significantly inhibit the tumor growth and
possess a synergistic antitumoral effect with the CDDP
. This effect caused a significant reduction in the serum
levels of aspartate transaminase, alanine transaminase
and creatinine in comparison to the positive control. These
results suggest that the methanolic extract of S.atrop
might contain phytoconstituents endowed with antitumoral
properties, which could protect against the colorectal
cancer.