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Pharmacognosy 2019
March 11-12, 2019
London, UK
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 57
Pharmacognosy and
Medicinal Plants
7
th
Edition of International Conference on
Am J Ethnomed 2019, Volume 6
DOI: 10.21767/2348-9502-C1-009
Chemical study, antioxidant analysis and evaluation of the
larvicidal potential against
Aedes aegypti
larvae of essential
oil of
Ocimum basilicum
Linn.
Sheylla S M S Almeid, Rosany L Martins, Ana L F Farias, Alex B L Rodrigues
and
Érica M Rabelo
UNIFAP, Brazil
T
he purpose of this research was to accomplish
chemical study, antioxidant analysis and evaluation
of the larvicidal potential against
Aedes aegypti
larvae
of essential oil from the leaves of
O. basilicum
Linn. The
research was carried out in the Pharmacognosy and
Phytochemistry Laboratory, Department of Biological and
Health Sciences, Federal University of Amapá (UNIFAP),
between July 2013 and March 2014. Arthropoda
Laboratory, Department of Biological and Health
Sciences, Federal University of Amapá (UNIFAP) between
September 2013 and March 2014. The essential oil
was obtained by hydrodistillation; the identification and
quantification of components was achieved with the use
of GC-MS analysis.The antioxidant activitywas evaluated
by themethod of sequestration of DPPH. The essential oil
was tested in the third larval state of the development of
the mosquito
Aedes aegypti
. The third larval instar were
exposed to different concentrations of the oil (500, 400,
300, 200 and 130 ppm) in triplicates. Chromatographic
analysis identified that the major constituents found
in essential oil of
O. basilicum
were limonene (13%),
1,8-cineole (15%), linalool (20%) and methyl chavicol
(45%). In trials of free radicals sequestration, the
essential oil showed (AA%) 67.35±1.11 in the highest
concentration and inhibitory concentration, IC50 value of
61.517 mg/mL. The essential oil of
O. basilicum
showed
larvicidal potential with CL50 of 67.22 ppm. A more
detailed study should be done to verify the larvicidal
potential and biological mechanism of action, as several
authors claimed that the constituent of essential oils
affect the nervous system of the mosquito
Aedes aegypti
and the actionmechanism is not yet fully elucidated. New
studies demand the development of tests using samples
of lower concentrations to verify the degree of toxicity in
other animal species, including man and preparation of
formulations that may function as a natural alternative to
combat mosquito larvae.