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Pharmacognosy 2018 American Journal of Ethnomedicine
ISSN: 2348-9502 Page 31
April 16-17, 2018
Amsterdam, Netherlands
6th Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Medicinal plants have been found promising in treating
diseases throughout the world. Herbal treatments are
preferred over synthetic drugs due to fewer side effects
as reported otherwise in terms of adverse drug reactions,
drug-drug interactions and drug resistance so far. Pakistan
has plenty of natural resources and is well known for their
diverse and valuable medicinal plants. Berberis lyceum is a
highly medicinal plant present widely in Pakistan and other
countries. In this study, methanol and ethanol extracts of
root, stem and leaves of Berberis lyceum were extracted
through Soxhlet method. Alkaloids were isolated through high-
performance liquid chromatography (HPLC) by using SilC18
column with acetonitrile and potassium dihydrophoshphate
as mobile phase. The elution rate was 1.0 ml/min and the
detection was monitored at 346 nm. High-performance liquid
chromatography (HPLC) analysis showed berberine in crude
extract of stem but root crude extracts contain high amount
of berbamine and low amount of berberine, while leaf extracts
showed negative results. Insecticidal activities in this study
against the most common and pest resistant insect, Tribolium
castaneum, showed good results with 20% concentration of
ethanolic and methanolic crude extracts of Berberis lyceum
with malathion combination. The insects were tested with 20%
methanolic and ethanolic extracts of root stem and leaves
alone and combination of extract with different concentrations
of malathion. Combination of extracts of stem and root showed
maximum mortality rate as compared to crude extracts alone.
Our extracts do not actually kill the insects; it can make them
sensitive to any insecticide. After applying our extracts, insects
can be killed from any insecticide, but leaf extracts again
showed no promising results.
Characterization and determination of chemicals in Sumbul
(Berberis lyceum Royle) through HPLC and insecticidal activities
against common resistant insect Tribolium castaneum
Humaira Shaheen
COMSATS Institute of Information Technology, Pakistan
Humaira Shaheen, Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-005