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Pharmacognosy 2018 American Journal of Ethnomedicine
ISSN: 2348-9502 Page 29
April 16-17, 2018
Amsterdam, Netherlands
6th Edition of International Conference on
Pharmacognosy and
Medicinal Plants
Statement of the Problem: Polyunsaturated fatty acids (PUFAs)
have been the subject of research due to their nutritional and
physiological roles. The family, Boraginaceae Juss. is one of
the sources of PUFAs. The lipids of seeds Pulmonaria mollis
Wulfen ex Hornem of this family, widespread in the flora of
Russia, have not previously been studied.
Methodology & Theoretical Orientation: The object of the
study was mature seeds of wild Pulmonaria mollis from
different climatic zones - Adygea (1) and Bashkortostan (2).
Oil from seeds was extracted using the Soxhlet’s procedure.
Separation and identification of lipid classes was performed
using a silica column chromatography, TLC and preparative
TLC. The FA composition was determined by converting into
FA methyl esters followed by GLC.
Findings: Oil content (%, seed weight) in samples was 33.8
and 19.1%, respectively, content of polar lipids was 0.4 and
0.9, including glycolipids was 0.4 and 0.5 and phospholipids
was 0.1 and 0.4. The NL subclasses consisted of sterol
esters – 1.7 and 1.7%, triacylglycerols – 92.2 and 86.4, free
fatty acids – 0.8 and 6.4; diacylglycerols+ sterols – 3.2 and
3.2; monoacylglycerols – 2.2 and 2.3. FA composition 1 and 2
did not differ. Palmitic acid was the most abundant among the
saturated FA (about 31%). Oleic acid was the single dominating
among monoenoic FA. The PUFAs found were dominated by FA
ω-6 (18:2 linoleic acid up 32%, 18:3 gamma-linolenic acid up
19%) andω-3 (18:3alpha-linolenic acidup15%, 18:4 stearidonic
acid up 5%). The amounts of PUFAs in NL were about 65%. The
PUFAs was concentrated by the urea complexation method.
The total concentration of PUFAs increased by up to 63% % of
ω-6 PUFAs and 29% ω-3 PUFAs.
Conclusion & Significance: Pulmonaria mollis seeds could be
considered potential additional sources of PUFAs and the plant
is promising for its introduction.
Recent Publications
1. Yunusova S, Lyashenko S, Fedorov N and Yunusov M
(2017) Lipids and lipophilic constituents of comfrey
(Symphytum Officinale L.) seeds. Pharmaceutical
Chemistry Journal 50:728–731.
2. Guil Guerrero J, Gómez-Mercado F, Ramos-Bueno
R and González-Fernández М (2017) Sardinian
Boraginaceae are new potential sources of gamma-
linolenic acid. Food Chem. 218:435–439.
3. Guil Guerrero J, Gómez-Mercado F, Ramos-Bueno
R and Rincón-Cervera М (2014) Restricted-range
Boraginaceae species constitute potential sources of
valuable fatty acids. J. Am. Oil Chem. Soc. 92:301–308.
4. Yunusova S, Khatmulina L, Fedorov N and Ermolaeva
N (2012) Polyunsaturated fatty acids from several
plant species of the family Boraginaceae. Chem. Nat.
Compd. 48:361–366.
5. Yunusova S, Yunusov M, Karimova A and Mironov
V (2007) Lipids of oenothera seeds from different
habitats. Chem. Nat. Compd. 43:525–528.
Biography
S S Lyashenko is a candidate of Pharmaceutical Sciences (PhD in Pharmaceutical
Sciences), and Senior Lecturer in the Department of Pharmacy Faculty of Post-
graduate Education, Рyatigorsk Medical Pharmaceutical Institute of Volgograd
Medical State University. In 2011 she defended her thesis on “Pharmacognosy
study of Borage officinalis L.”. Since several years (2008 to 2017), she has been
performing fragments of scientific work at the Laboratory of Bioorganic Chemis-
try and Catalysis, Institute of Chemistry, Ufa Scientific Center, Russian Academy
of Sciences. As a Researcher, she searches and analyzes plant sources of ω-6
and ω-3 polyunsaturated fatty acids, as well as studies of lipophilic components,
exhibiting high biological activity. She investigates promising species of the Rus-
sian Federation for its introduction, particularly the family Boraginaceae Juss.
Pulmonaria mollis Wulfen ex Hornem seed oil—a promising
vegetarian source of polyunsaturated fatty acids
S S Lyashenko1, S G Yunusova2, Z Yu Tleptserisheva1, O N Denisenko1, M S
Yunusov 2 and N I Fedorov 2
1Volgograd Medical State University, Russia
2Russian Academy of Sciences, Russia
S S Lyashenko et al., Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-005