11 / 38
Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 30
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
T
heobjectiveof thisstudywas to investigate theacute toxicity
and potential activity of methanolic extract of Hyoscyamus
muticus (Me-HM) to assess nociception in mice. The acute
toxicity was studied in both oral and intraperitoneal route. LD50
was determined using Probit method and the effect of extract
against nociceptive was studied by thermal stimulus (hot
plate) and injection of chemical substances such as formalin
(formalin test) and acetic acid (writhing test). Morphine was
used as positive drug in hotplate test and acetylsalicylic acid
was used in formalin and writhing test. The antinociceptive
activity was determined by observed increase of latency time
in hotplate test, decrease of abdominal constriction in writhing
test and decrease of stretching in formalin test. The LD50 of
intraperitonial administration of Me-HM was 1000±42, 89 mg/
kg-1. Our extract produced a significant (P≤0.001) and dose
dependent increase of latency time in hotplate test. The optimal
effects were observed after 90 mins of oral administration
of both doses. In the formalin test, the both doses reduce
significantly (P≤0.001) the effect produced by intraplantar
injection of formalin with maximum inhibition recorded in
neurogenic phase with 49.36% and 42.67% successively for
100 and 50 mg/kg of Me-HM. Morphine and acetylsalicylic
acid produced desired anti-nociceptive activity in tests used
in this study. The antinociceptive effect of H. muticus extract
can be explained probably by binding of scopolamine and
hyoscyamine from extract to muscarinic receptors and 5- HT3
involved in pain pathways. It was concluded that Me-HM shows
a remarkable antinociceptive activity in thermal and chemical
model of nociception in mice
hicham221287@gmail.comAcute toxicity and anti-nociceptive activity of methanolic
extract of Hyoscyamus muticus in mice
Hicham Boufous
1
, Fatimazahra Marhoume
1
, Abderrahman Chait
2
and
Abdellah Bagri
1
1
Hassan 1
st
University, Morocco
2
Cadi Ayyad University, Morocco
Hicham Boufous et al., Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-005