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Pharmacognosy 2018
American Journal of Ethnomedicine
ISSN: 2348-9502
Page 111
April 16-17, 2018
Amsterdam, Netherlands
6
th
Edition of International Conference on
Pharmacognosy and
Medicinal Plants
D
enguehasremainedtobeanationalepidemicinthePhilippines
and has been one of the leading causes of mortality in
children. Although there is no quick dengue infection confirmation
test, a complete blood count might show thrombocytopenia and
leukopenia.
Syzygium cumini
(L.) Skeels (Myrtaceae) is widely
used for various diseases and is particularly abundant with
quercetin. The air-dried S. cumini leaves underwent sequential
extraction using hexane, ethyl acetate, and methanol followed
by phytochemical screening, where the methanolic extract was
found to contain the highest amount of flavonoid (87.5 mg QE/
gram), followed by the ethyl acetate extract (56.1 mg QE/gram),
and the hexane extract (32.5 mg QE/gram), respectively. The
methanolic leaf extract, given its numerous toxicity studies to be
safe from 5 to 2,000 mg/kg as per OECD 423 guidelines, was then
subjected to
in vivo
bioassay utilizing 24 male Sprague-Dawley
rats. The rats were divided into four groups (n=6) namely: distilled
water 10mL/kg p.o., hydroxyurea 15 mg/kg p.o., methanolic
extract 400 mg/kg p.o., and methanolic extract 800 mg/kg
p.o., where hydroxyurea was used to induce thrombocytopenia
and leukopenia in all groups. The thrombocyte and leukocyte
counts were measured before induction to get the baseline, after
induction, and at the 1st, 3rd, and 6th day of treatment. Applying
one-way ANOVA and Duncan Test as post-hoc, results revealed
that the methanolic leaf extract of S. cumini exerted an anti-
thrombocytopenic property at both doses of 400 and 800 mg/kg
and an anti-leukopenic property at the dose of 800 mg/kg. This
study significantly claimed for the first time that the leaves of S.
cumini canbeorally activeandeffective in increasingplateletsand
WBCs in hydroxyurea-induced thrombocytopenia and leukopenia.
Hence, it is a potential candidate for further research leading to
the development of an herbal therapeutic agent for dengue.
bandiolateresamayb@gmail.comAnti-thrombocytopenic and anti-leukopenic properties of
Syzygium cumini (L.) Skeels (Myrtaceae) leaves in a Murine model
Teresa May B Bandiola and Mary Jho-Anne T Corpuz
University of Santo Tomas, Philippines
Am J Ethnomed 2018, Volume 5
DOI: 10.21767/2348-9502-C1-006