nuclear-medicine-radiation-therapy-2018-posters

Page 57

Journal of Medical Physics and Applied Sciences

ISSN: 2574-285X

I n t e r n a t i o n a l C o n f e r e n c e o n

Nuclear Medicine &

Radiation Therapy

Nuclear Medicine & Radiation Therapy 2018

O c t o b e r 0 1 - 0 2 , 2 0 1 8

S t o c k h o l m , S w e d e n

any ligands such as diethylenetriaminepentaacetic acid (DTPA), glucoheptonate (GHA), a seven-carbon carboxylic acid

sugar, glucuronic acid (GA), a six-carbon carboxylic acid sugar bind with reduced radioactive metal

99m

TcO4- (Pertechnetate).

These ligands are assessed as radiotracer for nuclear renal imaging to diagnose the various diseases. But this radiotracer takes

prolonged time to accumulate in the kidney which results inwaiting for long time for the patients’ examination. Hence, development

of a new renal radiotracer is urgently required to reduce the time for the examination. On the other hand, N-acetyl neuraminic acid

(Neu5Ac) is a nine-carbon carboxylic acid monosaccharide (sialic acid) performs multiple functions in living cells. We developed

99m

Tc-radiolabeled Neu5Ac by direct labelling method using SnCl2.2H2O as a reducing agent. Factors such as amount of Neu5Ac,

pH, amount of radioactivity, reaction time and various quantities of reducing agent have been systematically studied to optimize

the radiochemical yield of complex. Further, we characterized the developed radio-complex by using various techniques that

includes ITLC; paper electrophoresis, HPLC, plasma protein binding, lypophilicity and

in vitro

serum stability at physiological

conditions. Investigation of coordinated technetiumwith Neu5Ac was done by cold rhenium using FTIR. Neu5Ac was successfully

radiolabelled with

99m

Tc as evidenced by high labelling efficiency more than 90%, radiocomplex showed partial negative charge

as it shifted toward anodic side.

In vitro

stability was 8 hrs in rat serum. Plasma protein binding is 43±3.4% compared to

131

I-OIH,

which has protein binding of 44%. Higher accumulation (%ID/g) of radiotracer was observed in kidney, however liver and spleen

appears first 15 min and rapidly cleared. Scintigraphic images also reflect the same pattern of radiotracer uptake as observed

in bio-distribution studies in rat. Finally this radiocomplex could be converted as new radiopharmaceutical after rigorous quality

control in diagnostic role for renal imaging in nuclear medicine field.

99mTc labelled N-Acetyl neuraminic acid as a

new radiotracer for renal imaging preparation

and preclinical study

Ravi R kumar

, Dewinder K dhawan

and Vijayta D chadha

Centre for Nuclear Medicine (U I E A S T), Panjab University, India

Department of Biophysics, Panjab University, India

J. med phys & appl sci 2018, Volume: 3

DOI: 10.21767/2574-285X-C1-003