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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320
Page 84
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2
n d
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
T
ype 1 diabetic mellitus (T1DM) is known to be associated
with progressive destruction of β-cells of the pancreas.
Dysregulated immunity and programmed cell death are an
important link in pathogenesis of diabetes. In this study, we
examined expression levels of
interleukin-2
,
BCL1
,
ANXA-11
genes in patients with T1DM. The study was done with blood
leukocytes of 30T1DMpatients and 70 healthy controls. Reverse-
transcription PCR was done with Transcriptor first strand cDNA
synthesis kit fromRoche Life Science. The study of IL-2 and BCL1
gene expression level in peripheral blood leukocytes indicated
that the median gene expression levels of
IL-2
and
BCL1
are
increased in patients with T1DM patients compared with control
group of healthy persons. The study of apoptosis by annexin test
has revealed an increased level of
ANXA-11
expression in T1DM
patients. The obtained data can serve as an additional source for
understanding the pathogenesis of T1DMmechanisms.
hzang@mail.ruApoptosis of immune cells in type 1 diabetes mellitus
Hovsepyan L M
1
, Kazaryan G S
1
, Zanginyan A V
1
, Akopdzhanyan A A
1
, Zakharyan
G V
1
,
and
Petrek M
2
1
Institute of Molecular Biology - NAS, Republic of Armenia
2
Palacky University, Czech Republic
J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2471-8084-C1-003