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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320
Page 80
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2
n d
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
T
he cell lines of
Aedes albopictus
(C636) obtained at
NIMHANS, Karnataka, India are maintained in incubators with
M&M insect cell culture medium and BSA to establish
Wolbachia
infections. The cell lines were inoculated with
Wolbachia
(A,
B and AB super groups) and the strength of the infection was
calculated based on the perpetuation of the cells in the
in vitro
medium. The comparison between control and infected cell
lines suggests a two-fold decrease in infected cells reaching
confluence. The inoculum strength differs from the source
of
Wolbachia
isolated from different insect hosts.
Wolbachia
extracts with double infections (AB) are highly virulent than single
infections. Among single infections,
Wolbachia
B super-group is
more virulent than A super-group. Further, it was observed that
Wolbachia
derived from
Exorista sorbillans
,
Aedes albopictus
,
Trichogramma japonicum
has greater virulence and cell lines
can be infected within few passages.
Wolbachia
isolated from
Talicada nyseus did not induce any significant effect on mosquito
cell lines. The findings of the current study append the database
of potential non-native
Wolbachia
strains that can be introduced in
mosquitoes for expression of novel phenotypes. Recent findings
reports increased virulence of pathogenic West Nile Virus, when
confounded with native
Wolbachia
strains in mosquitoes. Thus
screeningofalternative
Wolbachia
strainsthatcouldbemaintained
in mosquito cell lines and establishing a
Wolbachia
strain pool for
mosquito trans-infection is of significant importance.
puttarajuhp@hotmail.comDifferential proliferation of Wolbachia infections in
Aedes mosquito cell lines
Puttaraju H P
Bangalore University, India
J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2471-8084-C1-003