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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320 Page 82
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2 n d E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2 t h E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
Holarrhena pubescens belongs to the family Apocynacea,
commonly known as “kurchi” is highly reputed in traditional
medicine as a remedy for amoebic dysentery and other intestinal
ailment. Bioassay-directed fractionation by chromatographic
methods the ethanolic extract of Holarrhena pubescens resulted
in the isolation of steroidal alkaloids i.e. Holamide and Pubscinine
[1]. Holamide showed a three proton doublet at 1.45 (J=6.56 Hz)
and two AB doubles at 3.17 and 3.00 each for on proton (J=12.06
Hz) in the 1H NMR spectrum suggested that it belongs to
conanine series of alkaloid (A class of compound with the steroid
nucleus and a five members heterocyclic ring with nitrogen). In
contrast Pubscinine showed one methyl at 1.28 while the doublet
is missing a three proton singlet was observed at 2.28 due to a
vinylic methyl indicated a double bond in the 18,20 – epimino
ring of the conanine series of alkaloids. In anaesthetized rats,
the Holamide and Pubscinine caused a fall in blood pressure in
a dose-dependent manner. Pretreatment of animals Atropine
completely abolished the hypotensive response of Acetycholine;
whereas hypotensive effect of Holamide and Pubscinine were
not modified by Atropine [1]. Similarly Acetylcholine produced
contractile effect in guinea-pig ileum, which was antagonized
by atropine, however both (Holamide and Pubscinine) failed to
produced any stimulant response on guinea-pig ileum. These data
indicate that the steroidal alkaloids i.e. Holamide and Pubscinine
from Holarrhena pubescens mediated hypotensive response
through a mechanism different to that of Acetycholine.
Bioassay directed isolation of hypotensive alkaloids
from Holarrhena pubescens
K Aftab*1, S Khan2 , S B Usmani3, S Begum3 and B S Siddiqui3
1Sialkot Medical College, Pakistan
2Liaquat National Hospital Medical College, Karachi
3University of Karachi, Pakistan
J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2471-8084-C1-003