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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320 Page 76
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2 n d E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2 t h E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
In solid organs, there are the cells with regenerative potential.
They can start regeneration of the damaged tissue with a
support coming from the bone marrow. In the early 2000s, we
started a project in which the mononuclear cells of bone marrow
(BMMC) were used to save the legs of patients with critical limb
ischemia (CLI). Injection of the cells into the affected limb resulted
in several following events which started with a pain release than
healing of ulcerations and finally the shortening of a distance of
claudication. 40%of 23 patientswith CLI receiving BMMC enjoyed
remission lasted up to 10 years or more. We learned that several
subsets of BMMC may act in concert supporting each other in
achieving the goal revascularization of the limb. Revascularization
of the infarcted femur was achieved by implantation BMMC via
the holes drilled in the head of the femur. It appeared that the cells
with the MSCmarkers played rather a moderate role in preventing
the leg amputation as the main actors are endothelial progenitor
cells (EPC, CD391+) which are present in the implant. Similarly to
leukophoretic product BMMC also the marrow cells centrifuged
in own plasma gradient (commercially available kit) is enriched
in the cells having MSC and EPC markers. The cells of the latter
composition injected intra-articularly into the hip or knee brought
a relief in patients with osteoarthritis. We believe that the positive
effect of this cellular therapy is due to the presence in a fresh
fraction of BMMC a variety of progenitors including MSC and
EPC which acting in concert exert the anti-inflammatory, tissue
regenerative and angiopoietin effect.
Regenerative potential of CD45 negative marrow cells relies on
two separate subpopulations being either anti-inflammatory or
pro-angiogenic
Andrzej Lange
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy – PAS, Poland
J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2471-8084-C1-003