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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320
Page 68
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2
n d
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
T
he Schwann-like cells can be considered as promising in
stem cell therapies, at least in experimental models. Human
adipose-derived stem cells (ADSCs) are induced into Schwann-
like cells (SC-like cells) and are cultured on either a plastic surface
or laminin-coated plates. The findings here reveal that laminin is
a critical component in extracellular matrix (ECM) of SC-like cells
at
in vitro
. The survival rate of SC-like cells on a laminin matrix
are measured through MTT assay and it is found that this rate
is significantly higher than that of the cells grown on a plastic
surface (P < 0.05). Schwann cell markers and the myelinogenic
ability of SC-like cells at the presence versus absence of laminin
are assessed through immunocytochemistry. The analysis of
GFAP/S100β and S100β/MBP markers indicate that laminin
can increase the differentiated rate and myelinogenic potential
of SC-like cells. The expression levels of SCs markers, myelin
basic proteins (MBP), and neurotrophic factors in two conditions
are analyzed by real-time reverse transcription polymerase
chain reaction(RT-PCR).The findings here demonstrated that
gene expression of SCs markers, MBP, and brain-derived
neurotrophic factors (BDNF) increase significantly on laminin
compared to plastic surface (P < 0.01). In contrast, the nerve
growth factor(NGF) expression is down regulated significantly on
laminin-coated plates (P < 0.05). The obtained data suggest that
production of neurotrophic factors in SC-like cell in presence of
laminin can induce appropriatemicroenvironment for nerve repair
in neurodegenerative diseases.
Biography
Dr. Tadjalli is a professor of Histology and Zarinfard is a graduate student
at the department of Histology School of Veterinary Medicine, Shiraz Uni-
versity, Shiraz and Dr. Razavi is a professor of Histology Isfahan University
of Medical Science, Isfahan, Iran. Her research interest includes Histology,
stem cell, regeneration.
mtadjalli6@yahoo.comEffect of laminin on neurotrophic factors expression in
schwann-like cells induced from human adipose-derived stem
cells
in vitro
Mina Tadjalli
1
, Giti Zarinfard
1
and
Shahnaz Razavi
2
1
School of Veterinary Medicine, Shiraz University, Iran
2
School of Medicine, Isfahan University of Medical Sciences, Iran
Mina Tadjalli et al., J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2573-0320-C2-006