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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320
Page 67
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2
n d
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
L
ymphatic vessels play a crucial role in draining excess fluid
and transport macromolecular substances from extracellular
spaces. Disfunction of lymphatic vessels may cause lymph
edema and chronic inflammation, leading to fibrosis of the local
tissue. This study investigated efficiency of transplantation of
lymphatic endothelial progenitor cell (LEPCs) and sustained
release of VEGF-C from self-assembling peptide (SAP) on
promoting lymphangiogenesis after myocardial infarction (MI).
CD34+VEGFR-3+ EPCs were isolated from rat bone marrow.
Sustained release of VEGF-C from SAP nanofibers (SAPNs)
was detected with ELISA. Compatibility of SAPNs with the cells
was accessed with transmission electron microscopy and EB/
AO staining. After rat MI models were established with ligation
of the anterior descending branch of the left coronary artery,
SAP carrying the cells and VEGF-C was injected at the border
of the infarcted region. At four week after transplantation, the
survival and differentiation of the cells labeled with GFP were
examined, and repair of the infarcted myocardiumwas evaluated.
Under induction with VEGF-C, CD34+VEGFR-3+ EPCs could
differentiate into lymphatic endothelial cells. The cells spread
well along SAPNs. SAPNs protected the cells from apoptosis in
the condition of hypoxia, and released VEGF-C sustainedly. After
transplantation, cardiac function was improved significantly.
The number of the survived cells increased, and some cells
differentiated into lymphatic endothelial cells. Density of
lymphatic vessels increased, and cardiac edema was reduced.
Moreover, angiogenesis and myocardial regeneration were
enhanced. These results suggest that SAPNs load LEPCs and
release VEGF-C effectively. VEGF-C released fromSAPNs induces
differentiation of LEPCs towards lymphatic endothelial cells.
Loading stem cells and releasing growth factor with SAPNs is a
promised strategy for MI therapy.
References:
1. Wang Q L, Wang H J, Li Z H, Wang Y L, Wu X P and Tan Y
Z (2017) Mesenchymal stem cell-loaded cardiac patch
promotes epicardial activation and repair of the infarcted
myocardium. J Cell Mol Med. 21:1751–66.
2. Zhou P, Tan Y Z, Wang H J and Wang G D (2017) Hypoxic
preconditioning-induced autophagy enhances survival
of engrafted endothelial progenitor cells in ischaemic
limb. J Cell Mol Med. 21:2452–64.
3. Wang G D, Tan Y Z, Wang H J and Zhou P (2017)
Autophagy promotes degradation of polyethyleneimine–
alginate nanoparticles in endothelial progenitor cells. Int
J Nanomed. 12: 6661–75.
Biography
Hai-jie Wang is a Professor of Department of Anatomy, Histology and Em-
bryology at Shanghai Medical School of Fudan University. He studied Clini-
cal Medicine at Weifang Medical College, China. He completed his MD from
Medical School of Shandong University, China in 1987 and PhD from Shins-
hu University School of Medicine, Japan in 1996. He studiedMolecular Med-
icine at School of Medicine, Yale University from 2005 to 2006 as Visiting
Professor. In 1999, he became a Professor of Shanghai Medical School of
Fudan University. His research interests include “Differentiation and trans-
plantation of endothelial progenitor cells”.
hjwang@shmu.edu.cnLymphatic endothelial progenitor cells and VEGF-C loaded with
self-assembling peptide nanofibers promote lymphangiogenesis
in infarcted myocardium
Hai jie Wang, Hai-feng Zhang, Yu-zhen Tan
and
Yong-li Wang
Shanghai Medical School of Fudan University, China
Hai jie Wang et al., J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2573-0320-C2-006