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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320
Page 63
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2
n d
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
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t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
Anti smooth muscle antibodies (ASMA) and tumor necrosis
factor (TNF) in Iraqi patients infected with hepatitis C virus
Hadaf Dhafir El Yassin
1
and
Rana A Hadi
2
1
University of Baghdad, Iraq
2
Iraqi Board for Medical Specializations, Iraq
Hadaf Dhafir El Yassin et al., J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2573-0320-C2-006
Background:
Hepatitis C virus (HCV) is a serious infectious
disease that can cause lifelong infection. Infection with chronic
hepatitis C virus (HCV) can lead to autoimmune hepatitis
(AIH) in a minority of patients. A genetic predisposition to
autoimmune hepatitis frommedicationmay lead to appearance
of serum autoimmune antibodies especially anti smooth
muscle antibodies (ASMA). Viral infection induces tumor
necrosis factor (TNF-alpha) production in hepatocytes. These
findings suggest that both parameters may have an important
role in the patho-physiology and drug resistance of human liver
diseases induced by viruses.
Aim:
The aim of the presents study was to evaluate the role
of the immunoendocrine system in the pathogenesis of the
disease, by measuring serum TNF and antismoothmuscle
antibodies (ASMA).
Subject and methods:
Sixty- one chronic hepatitis C
patients were consequently selected from the Medical city,
Gastrointestinal Hospital in Baghdad, Iraq, during the period
from July 2014 to September 2014, their median age was 34.8
year, 29 of them were males and 32 were females. All patients
were diagnosed having positive for HCV RNA by means of
polymerase chain reaction. The study also included twenty
apparently healthy adult ages and sex matched considered
as controls, which were negatively screened with hepatitis C
virus. Peripheral blood sample of 2 ml was aspirated using
disposal syringes. Samples were collected between (9.00a.m-
12.00p.m.). The blood was allowed to clot in plain tube for
30-45 minutes at room temperature. Sera were obtained by
centrifugation of the collected blood and then stored in plain
tubes at -20°C. ELISA method was used to measure serum
TNF, while ASMA was measured by indirect immunofluoresent
assay.
Results:
The results of this study showed an increase in mean
value of serumTNF in chronic hepatitis C patients accompanied
with a 65% increase in ASMS. Significant correlations were
found between both parameters studied.
Conclusions:
Chronic hepatitis C is associated with an
immunological abnormality. Results obtained might shed a
light on the type of therapy and drug of choice when managing
the disease.
Biography
Prof. Dr. Hadaf Dhafir El Yassin was faculty in University of Baghdad in the
Department of Biochemistry, College of Medicine at University of Baghdad.
She finished her Post Doctorate in Clinical Biochemistry at Al-Nahrain Uni-
versity. She is currently the head of the Department of Clinical Biochemistry,
University of Baghdad, Iraq. She actively participated in 43 local conferences
in Iraq and 26 abroad, making a total of 69 conferences attendance and
paper presentation. She also published 85 articles.
hedefdhafir@yahoo.co.uk