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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320
Page 64
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2
n d
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
Introduction:
The major signaling transduction of the pro-
inflammatory cytokine IL-6 is through the transcription factor
STAT3. However, PI3-K/AKT signaling pathway can also activated
by IL-6 under prostate pathological conditions.
Objectives:
The aimof this study is toevaluate the tissues levels of
STAT3/IL-6/ AKT axis signaling in prostate tissues from patients
with benign prostatic hyperplasia (BPH) and prostate cancer (PC).
Material & Methods:
Immunohistochemical analyses for IL-6,
Gp130, phospho-STAT3 (Tyr705) and phospho-AKT (Ser473)
were carried out in 25 samples of BPH, 16 samples of PC.
Results:
Immunoreactivity to IL-6 was consistently observed in
stroma compartment of BPH and cytoplasmic epithelial cell in
PC samples. Phospho-AKT was mainly expressed in membrane
and the cytoplasm in PC compared to BPH. Immunoreactivity
for phospho-STAT3 (Tyr705) was found in the stroma and the
nucleus of epithelial and tumoral cells. No significant association
was determined (r=0.153,
P
=0.518) when IL-6 and phospho-AKT
(S473) were analyzed within BPH patients; whereas a positive
correlation emerged between phospho-STAT3 (Tyr705) in the
stroma and phospho-AKT (S473). In PC patients, significant
relationship was documented between IL-6 and phospho-AKT
(Ser473) (r=0.725,
P
=0.02). In addition, the correlation between
phospho-AKT (Ser473) and phospho-STAT3 (Tyr705) as well as
detected in the nucleus and the stroma were significant.
Conclusion:
This suggests that IL-6/AKT axis could be one of
mechanism to activate STAT3 by facilitating inflammatory cell
migration and chronic inflammation in BPH and promote cancer
progression by promoting cell growth in PC.
Biography
Yosra Bouraoui is experienced in immunology, inflammatory and prostate
cancer. Yosra worked on prostate tissue by immunohistochemistry to an-
alyze several signaling pathways in response to inflammatory cytokines
such as IL-6, IL-1 and TNF alpha. She is expertized in prostate cell culture
to study AKT signaling and NF kappa B mediated by IL1. At this moment
she is working on petri net model (In silico model) my experiment results on
pro inflammatory cytokines and its network in prostate pathologies. Yosra
is currently working on the relation between inflammatory cytokine and im-
mune metabolic in prostate tissue.
yosra_132001@yahoo.frThe immune profile of prostate epithelial cells by IL-6
activation mediated by STAT3 and AKT signaling pathways
Yosra Bouraoui
1, 2
, N Ben Rais
3
and
R Oueslati
1
1
Carthage University, Tunisia
2
Jendouba University, Tunisia
3
HMPIT, Tunisia
Yosra Bouraoui et al., J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2573-0320-C2-006